Answer / nabil

there are many resons for different RT
1-The pressure used(because that affects the flow rate of
the solvent)
2- The nature of the stationary phase(not only what
material is made of, but also particle size.
3-the exact compositionsof the solvent.
4-the temp of the column.

Answer / srinivas

many reaons for different RT in same column
1) Depend on PH in buffer Preparation
2) copmposition of buffer and solvent
3) difference in manual injector and auto injector

Answer / prabhakar

Because preparation of mobile phase variation(mostly)

Answer / dhaval bhatt

1)column temp
2)presser not stable
3)leakage column from any side

Answer / vaibhavgoswami

due to pressure ,
different column packing,(silica)
different maker

Answer / ravi kumar tummala

if mobile not sonicated properly the retention time will vary

Answer / phanindra

I think it is due to contamination of HPLC column remedy is column kept for wash with 100%polar solvent.

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