Question { 16395 }
If we are getting split peak in Chromatogram,what we can do?
Answer
Main cause : That means u r column is contaminated
absorbant ion of impurites in the column.
Solution :
1)Wash calumn:-By polar solvent -> semi polar solvent -> non
polar solvent ->semi polar solvent -> polar solvent.
2)Check column performance
3)Replace column