Answer / amol

because , when manufacturing of column is done different
parameter is affected which is given below , due to this
each column is unique tho it is same type.

column parameter

column pressure
parical size
carbon density
dead voulume of the column
end fitting
end capping of the column.
silica used.

Hplc System Parameter

length of tubing.
internal dia. of tube.
void volume of system.
pump working.
flow rate.
end fittings.
ETC....

Answer / satheesh kumar

In HPLC same type of columns means say C18,there are lot of
brands C18 columns available in market namely
inertsil,Symmetry,X-Terra,phenomenex gemini,Zorbax etc.,

Each columns are not similar in packing and carbon
load.column chemistries will vary with one another.

Hence RT shift observed in same types of columns.

Answer / reet

following factors might be the reason:
1. Column ageing
2. how the column was preserved, or what mobile phase was
used before the present experiment
3. sample preparation might be the cause. sample may be non-
homogeneous
4. Slight variation in thepH may also affect the variation
in RT.
5. Other parameters like,temperature can also be one of the
reason.
6. last but an imporatant factor might be some air bubble
trapped in the system.

Answer / ranjit

it is depends upon polarity of the colunm

Answer / ali

The difference in RT is usually caused by column age and how
it was conditioned before the analysis.

Answer / ravi.a

it is depend on used mobile phase is one of the reason if u
get peaks area are same if not get same areas that column
is dameged due to in colun stationary phaes coming ot side

what is the meaning of PURITY AND POTENCY .what is the difference between PURITY and POTENCY

5 Answers   Orchid,

why require the ph, buffer during hplc mobile phase?

0 Answers  

If we are getting split peak in Chromatogram,what we can do?

7 Answers  

why we multiply 1000 in the determination of kf factor ?

3 Answers   Stellence Pharm Science,

How to calculation of residual solvent methanol (3000 ppm) standard solution, and how many quantity used of test sample

0 Answers  

what is the difference between the ordinary loss on drying technique and vacuum oven technique?

1 Answers   Divis Laboratories,

what is use of acetonitrile compare to methanol in RP-HPLC Method devlopment

7 Answers   Neuland, Orchid,

What is Impuriteis?

4 Answers   Zydus Cadila,

what is the value of pH

11 Answers   Reddy Labs,

wt is difference between assay and purity in hplc?

9 Answers   Hetero, IDRS Labs,

how to regulate noise in HPLC?

2 Answers   Alkem Labs, Vimta Labs,

How to prepare 50 ppm ammonia solution form 25% ammonia solution?

8 Answers   Cadila, IIT,