Why the same type of columns showing different RT for a single
product?

Answer Posted / amol

because , when manufacturing of column is done different
parameter is affected which is given below , due to this
each column is unique tho it is same type.

column parameter

column pressure
parical size
carbon density
dead voulume of the column
end fitting
end capping of the column.
silica used.

Hplc System Parameter

length of tubing.
internal dia. of tube.
void volume of system.
pump working.
flow rate.
end fittings.
ETC....

Is This Answer Correct ?    20 Yes 2 No



Post New Answer       View All Answers


Please Help Members By Posting Answers For Below Questions

How do we fix the sample concentaryion in hplc method development. What is the basis?

682


why we are using hexane in calibration of number of drop per mL

1172


How to compare XRD graphs against standard and carry polymorpism study of API's by powder XRD method?

3396


what is definition of validation? which components are followed give detail?

1832


What is the acceptance criteria for moisture balance when calibrated with sodium sulfate

1553






before starting analytical method valodation what you checking? and how giving preference to start validation?

352


how can give the expiry period and restadardisation of volumetric solution

2056


process of Diclofenac sodium,IP.

1678


how you confirm the assay method?

293


How to set specification of assay, fisdolution and related substances?

370


in sampling of raw material which side is pasted sampled labels

1249


how to set sample and standard concentration in RS method?

366


How to calculate coreletion coefficient

1142


what is mean by nitrosamine impurities?

546


how a particular wavelength can be different for a particular compund while analysing by uv and by HPLC.

1838