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In GC and HPLC ,how we can decide which of following method will be suitable or use for quantitation of chromatogram at the time of method development. 1.Area Normalisation 2.External standard method 3.Internal standard method
Atul,
1 7203In capadity factor formula is K'= (RT of main compound/RT of first eluted peak)-1 in this formula what is 1
1 6981hii, i'm had a sample of 1L that gone through extraction & evaporation. i used 0.5ml of methanol for reconstitution before injecting the sample to HPLC. i made a calibration curve using a standard solution but now i'm confused about how to display the concentration i got from the sample. for ex. i got 0.2ug/ml how i'm gonna explane this is for the 1L ? please help .. i'll really appreciate it thanks
1 4131Dear Sir, This is Thirunavukkarasu am working in Ordain Health Care AR&D, Am Having one problem While doing method development my question Metadoxine and Pyridoxine same chemical nature and structural activity i tried lot of methods but Analyte eluted same Retantion time i cant separate both peaks. solubility, pH both are same only only difference melting point metadoxine 210 C Pyridoxine 102 C please do the help needful for me
5 9517some product having water content method with pyridine and ethylene glycol mixture instead of methanol? how they are selecting and methanol is not suitable solvent how ?
2600Post New Analytical Chemistry Questions
Basic principle of ESI?
why we are using benzene, anyline in acetic anhdride assay titration method?
cefoperozone and sulbactam inj. hplc test method
If we do accuracy at same concentration at which linearity planned,what is the need to do linearity separately?
identification is for unknown? qualification for known? reporting for LOQ?
What is stray light in uv what are units for stray light
in stress study if your api not soluble in hcl naoh h2o2 then what require to do?
For titration in anhydrous media with perchloric acide, if lack of titrator, Which indicator is been used for replacement. How calculate pH of test solution to choose suitable indicator?
function of detecter in hplc ,gc and spectroscopy? function of carrier gas in gc?
What is the Formula for coreletion coefficient in plhplc calibration
what is the structure of DOWEX-50
can i use hplc detector to uplc and why?
EXPLAIN THE CALCULATION PART OF AN KF TITRATOR CALIBRATION WITH SUITABLE EXAMPLE
1.What is the difference between method validation and method verification 2.Which guidelines proposed to method transfer
What is shaking level in GC?
