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What reaction is catalyzed by proteases? What steps are involved in determining the primary structure of a protein?
2 23198What are the differences and respective advantages to electrophoresis, SDS PAGE, and isoelectric focusing?
6 25576Where does ATP rank on a scale of phosphate transfer potential? What is the significance of this location?
3 8497I have given the protocol for the cyclodextrin glygosyl transferase assay: One ml of appropriately diluted enzyme sample was incubated at 60 °C for 15 min with 5 ml of 1% (w/v) gelatinized soluble starch in 50 mM, 7.0-pH Tris–HCl buffer. Reaction was terminated by boiling the reaction mixture for 3 min and reaction volume was made to 10 ml with distilled water. Two ml of above reaction mixture was withdrawn and mixed with 3 ml of Tris–HCl buffer, 5 ml of 125 mM Na2CO3, and 0.5 ml of phenolphthalein (25 mg phenolphthalein/100 ml absolute alcohol). Absorbance was measured at 550 nm. The percent decrease of sample was calculated with respect to control containing 5 ml of buffer, 5 ml of sodium carbonate and 0.5 ml of phenolphthalein. where Acontrol = absorbance of control and Atest = absorbance of sample. The amount of β-cyclodextrin (β-CD) produced was estimated from the standard graph of 0–500 μg/ml β-CD concentration against % decrease in absorbance. One unit of CGTase was defined as the amount of enzyme required to produce 1 μm of β-CD/min. Please can you suggest me the formula for the defination given in the last line
2305Post New Bio Chemistry Questions
Name a sulphur containing amino acid.
What is saytzeff’s rule?
Which is the least abundant Alkaline earth element?
Polymers of vinyl chloride are called?
Do enzymes act better under acid or basic ph?
Explain which type of chemical bond maintains the pairing of each chain in the dna molecule?
Which oxo acids of sulphur contain s-s bonds?
What is xylose?
How is the cooling of organs and tissues for medical transplantations associated with the effect of temperature upon enzymatic reactions?
Which group of elements is called alkaline earth metals?
What are dextrorotary compounds and levi rotary compounds?
why we use 96 well plate in ELISA plate reader?
What is the numeric relation between pyrimidine and purine bases in the dna molecule? Is that relation valid in rna molecules?
Why does physical exertion increase symptoms of poisoning by trematol?
what is your aspiration in profession/ career?
