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What reaction is catalyzed by proteases? What steps are involved in determining the primary structure of a protein?
2 23077What are the differences and respective advantages to electrophoresis, SDS PAGE, and isoelectric focusing?
6 25379Where does ATP rank on a scale of phosphate transfer potential? What is the significance of this location?
3 8362I have given the protocol for the cyclodextrin glygosyl transferase assay: One ml of appropriately diluted enzyme sample was incubated at 60 °C for 15 min with 5 ml of 1% (w/v) gelatinized soluble starch in 50 mM, 7.0-pH Tris–HCl buffer. Reaction was terminated by boiling the reaction mixture for 3 min and reaction volume was made to 10 ml with distilled water. Two ml of above reaction mixture was withdrawn and mixed with 3 ml of Tris–HCl buffer, 5 ml of 125 mM Na2CO3, and 0.5 ml of phenolphthalein (25 mg phenolphthalein/100 ml absolute alcohol). Absorbance was measured at 550 nm. The percent decrease of sample was calculated with respect to control containing 5 ml of buffer, 5 ml of sodium carbonate and 0.5 ml of phenolphthalein. where Acontrol = absorbance of control and Atest = absorbance of sample. The amount of β-cyclodextrin (β-CD) produced was estimated from the standard graph of 0–500 μg/ml β-CD concentration against % decrease in absorbance. One unit of CGTase was defined as the amount of enzyme required to produce 1 μm of β-CD/min. Please can you suggest me the formula for the defination given in the last line
2236Post New Bio Chemistry Questions
What is kc?
How do hydrophobic and hydrophilic residues arrange themselves in globular proteins?
The elution volume of an enzyme on a gel filtration column can be predicted from a) its enzyme activity b) its protein absorbance at 280 nm c) subunit composition and monomer molecular mass(es) d) Choices a) and b) are both correct.
According to the watson – crick model how many polynucleotide chains does a dna molecule has?
why life span of RBC IS GREATER THEN THE WBC?
Why is not it correct to assert that dna self-replicates?
What are respectively some remarkable functions of reverse transcriptase?
Explain what is the name of the dna duplication process? What is the main enzyme that participates in it?
What are respectively some remarkable functions of myosin, cd4, albumin, keratin, immunoglobulin, reverse transcriptase, hemoglobin, and insulin?
Why can it be said that the enzymatic action is highly specific?
why we use 96 well plate in ELISA plate reader?
What are the favourable conditions for formation of anions?
Concerning their biological function what is the difference between rna and dna?
What is the molecular formula, structure, and bond angle of phosphorous?
Who proposed law of mass action?
