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What reaction is catalyzed by proteases? What steps are involved in determining the primary structure of a protein?
2 22919What are the differences and respective advantages to electrophoresis, SDS PAGE, and isoelectric focusing?
6 25142Where does ATP rank on a scale of phosphate transfer potential? What is the significance of this location?
3 8178I have given the protocol for the cyclodextrin glygosyl transferase assay: One ml of appropriately diluted enzyme sample was incubated at 60 °C for 15 min with 5 ml of 1% (w/v) gelatinized soluble starch in 50 mM, 7.0-pH Tris–HCl buffer. Reaction was terminated by boiling the reaction mixture for 3 min and reaction volume was made to 10 ml with distilled water. Two ml of above reaction mixture was withdrawn and mixed with 3 ml of Tris–HCl buffer, 5 ml of 125 mM Na2CO3, and 0.5 ml of phenolphthalein (25 mg phenolphthalein/100 ml absolute alcohol). Absorbance was measured at 550 nm. The percent decrease of sample was calculated with respect to control containing 5 ml of buffer, 5 ml of sodium carbonate and 0.5 ml of phenolphthalein. where Acontrol = absorbance of control and Atest = absorbance of sample. The amount of β-cyclodextrin (β-CD) produced was estimated from the standard graph of 0–500 μg/ml β-CD concentration against % decrease in absorbance. One unit of CGTase was defined as the amount of enzyme required to produce 1 μm of β-CD/min. Please can you suggest me the formula for the defination given in the last line
2156Post New Bio Chemistry Questions
What are respectively some remarkable functions of reverse transcriptase?
What are some factors that can lead to protein denaturizing?
Which oxo acids of sulphur contain s-s bonds?
Explain what is the name of the dna duplication process? What is the main enzyme that participates in it?
What is the property of Alkaline earth metal Ions?
Why nitrogen cannot form penta halides?
why we use 96 well plate in ELISA plate reader?
What are pentoses? To what organic group do pentoses belong?
Differentiate between gram +ve and gram -ve bacteria.
What is nucleophilic addition reaction?
define standard feat of formation?
Who proposed VSEPR theory? What does it explain?
Because of dna replication, two dna molecules come to existence. Why is not it correct to assert that two “new” dna molecules are created? What is the name given to the process concerning that fact?
What are respectively some remarkable functions of hemoglobin?
What are the important sources of phosphorus?
