What types of changes can be used to effect fractional
precipitation of proteins?
Answers were Sorted based on User's Feedback
Answer / vipul panchal
various parameters r available.
1) pH- by using various pH change we can precipitate proteins having pI in the same range/
2)organic solvent- since each protein varies in their proportion of hydrophilic to hydrophobic we can make the use of increasing conc. of organic solvent thr by making the use of dielectric constant to isolate the proteins.
Is This Answer Correct ? | 0 Yes | 0 No |
Answer / vipul panchal
various parameters r available.
1) pH- by using various pH change we can precipitate proteins having pI in the same range/
2)organic solvent- since each protein varies in their proportion of hydrophilic to hydrophobic we can make the use of increasing conc. of organic solvent thr by making the use of dielectric constant to isolate the proteins.
3) Temperature- since some proteins are heat resistant this parameter can be use to isolate such protein.
4) salt conc.- by gradually increasing salt conc. we can fractionate the different proteins since depending upon their composition they differ in their solvation energy which can be change by changing ionic strength of solution.Process involves concept of salting out and salting in.
Is This Answer Correct ? | 0 Yes | 0 No |
Answer / rahul
salting out- add salts of higher ionic strength
decrease sulubility by adding organic solvents..
Is This Answer Correct ? | 0 Yes | 1 No |
Where is lactose mainly produced in mammals?
What are Tetracyclines?
3 Answers Dr Reddys, Reddy Labs,
Explain the basic structure of collagen?
What does law of mass action states?
Reduction of which intermediate in glycolysis leads directly to a compound involved in the biosynthesis of fats?
How do hydrophobic and hydrophilic residues arrange themselves in globular proteins?
What happens in methylation of dna?
what will happen to amixture of monomeric alpha and beta chains of haemoglobin at neutral ph and at 25 degree centigrade?
what is ph?
What is the difference between myoglobin and haemoglobin in the sequence of poly peptides?
explain the working of monochromator unit?
what is the test which is specific for tyrosine?