Interested to Buy Any Domain ? << Click Here >> for more details...
What reaction is catalyzed by proteases? What steps are
involved in determining the primary structure of a protein?
- 2 Answers
- 23187 Views
- I also Faced
- E-Mail Answers
Answers were Sorted based on User's Feedback
Answer / rajesh
A protease is any enzyme that conducts proteolysis; that is,
begins protein catabolism by hydrolysis of the peptide bonds
that link amino acids together in the polypeptide chain.
Classification
Proteases are currently classified into six groups:
* Serine proteases
* Threonine proteases
* Cysteine proteases
* Aspartic acid proteases (e. g., plasmepsin)
* Metalloproteases
* Glutamic acid proteases
| Is This Answer Correct ? | 14 Yes | 5 No |
Answer / rajesh
A protease is any enzyme that conducts proteolysis; that is,
begins protein catabolism by hydrolysis of the peptide bonds
that link amino acids together in the polypeptide chain.
Classification
Proteases are currently classified into six groups:
* Serine proteases
* Threonine proteases
* Cysteine proteases
* Aspartic acid proteases (e. g., plasmepsin)
* Metalloproteases
* Glutamic acid proteases
steps in determining the primary protein:
1.obtain a pure protein
2.determine the aminoacid composition and M.Wt of
proteins,from this calculate no. of aminoacid residues
present per protein molecule to the nearest whole number.
3.Reduce disulfide groups to sulfhydryl groups.
4.determine N-terminal and c-terminal.
5.fragment aliquots of polypeptide by enzymatic or chemical
hydrolysis and seperate peptide mixtures into individual
peptide fragments.
6.sequence each fragment,and by aanalysing the overlapping
parts,assemble the sequence of original protein.
| Is This Answer Correct ? | 6 Yes | 6 No |
What are the types of chromatography?
What are some factors that can lead to protein denaturizing?
Which property among the following generally increases from top to bottom in a group 1a? 1. Electropositive, density, basic nature, classical reactivity
how the zwitter ion formation takes place in aminoacid metabolism?
What does thermodynamics helps in predicting?
HOW PAPAVERINE CAN BE PREPARED FROM TYROSINE/
What is racemic mixture? Why it is optically inactive?
A principal difference between prokaryotic and eukaryotic DNA replication is a) completely different proteins/enzymes in eukaryotes b) multiple origins in eukaryotes c) no requirement for topoisomerase activity in prokaryotes d) the absence of a nucleus in prokaryotes
How do you define the molecularity of a reaction?
Why is it necessary to cleave proteins with at least two different reagents during sequencing?
Explain which type of chemical bond maintains the pairing of each chain in the dna molecule?
Explain what are the raw materials used for the precipitation of na2co3 by solvay ammonium process?
Micro Biology 
