Interested to Buy Any Domain ? << Click Here >> for more details...
Why nitrogen cannot form penta halides?
Name the vinyl halide that reacts with hydrogen halides and the product formed is?
What is the structural formula of molybdenum blue?
What is lattice energy?
What is the element in third series the element with maximum oxidation state?
In which oxidation state chromium exhibit different color?
How the production of rna called? What is the enzyme that catalyzes the process?
What are dextrorotary compounds and levi rotary compounds?
Why is the t-butyloxycarbonyl protecting group such a good choice for amino acid synthesis?
What is nucleophilic addition reaction?
Why does the pH of the blood decrease in a person who has digested trematol?
What is isomerism?
One characteristic of the dna molecule is its replication capability. What are the consequences of failures during dna replication?
Do enzymes act better under acid or basic ph?
I have given the protocol for the cyclodextrin glygosyl transferase assay: One ml of appropriately diluted enzyme sample was incubated at 60 °C for 15 min with 5 ml of 1% (w/v) gelatinized soluble starch in 50 mM, 7.0-pH Tris–HCl buffer. Reaction was terminated by boiling the reaction mixture for 3 min and reaction volume was made to 10 ml with distilled water. Two ml of above reaction mixture was withdrawn and mixed with 3 ml of Tris–HCl buffer, 5 ml of 125 mM Na2CO3, and 0.5 ml of phenolphthalein (25 mg phenolphthalein/100 ml absolute alcohol). Absorbance was measured at 550 nm. The percent decrease of sample was calculated with respect to control containing 5 ml of buffer, 5 ml of sodium carbonate and 0.5 ml of phenolphthalein. where Acontrol = absorbance of control and Atest = absorbance of sample. The amount of β-cyclodextrin (β-CD) produced was estimated from the standard graph of 0–500 μg/ml β-CD concentration against % decrease in absorbance. One unit of CGTase was defined as the amount of enzyme required to produce 1 μm of β-CD/min. Please can you suggest me the formula for the defination given in the last line
