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hii, i'm had a sample of 1L that gone through extraction & evaporation. i used 0.5ml of methanol for reconstitution before injecting the sample to HPLC. i made a calibration curve using a standard solution but now i'm confused about how to display the concentration i got from the sample. for ex. i got 0.2ug/ml how i'm gonna explane this is for the 1L ? please help .. i'll really appreciate it thanks
1 4306Dear Sir, This is Thirunavukkarasu am working in Ordain Health Care AR&D, Am Having one problem While doing method development my question Metadoxine and Pyridoxine same chemical nature and structural activity i tried lot of methods but Analyte eluted same Retantion time i cant separate both peaks. solubility, pH both are same only only difference melting point metadoxine 210 C Pyridoxine 102 C please do the help needful for me
5 9919some product having water content method with pyridine and ethylene glycol mixture instead of methanol? how they are selecting and methanol is not suitable solvent how ?
2708When ever we perform system suitability,inject the corresponding standard 6 times why? is thair any reson
4 10936Ratio of Water and Acetonitrile(diluent) is not recommended to use in cleaning method validation,why?
30331)What's the meaning of Absorption,give a example. 2)What's the meaning of Adsorption,give a example. 2)what is the difference between Absorption and Adsorption.
2949
what is classification of elemental impurities? what is risk assement in elemental impurities?
what is mean by peak intigrity?
What is colorimetry?
Describe the role of Protactinium in neutron sources and its significance for research in nuclear physics.
In BP2013, Loperamid HCl monograph. Assay by titration with 0.1N sodium hydroxide using hydrocloric acid 0.01N and reading the volume added between the 2 points of inflexion. I have a question that if the diluent solvent is ethanol is certainly consumed a amount of volume of titrant, so this volume must be eliminated on the result calculation or not apart from first point which is subtracted above.
How will u perform degradation study in detectors other thann PDA in HPLC Methodology
What is the range of uv & vis lamp in spectrophotometer?
in hplc chromatogram started from left to right and in uv spectrum started from left to right
If change in specification which parameter require to do for validation? if change in chromtographic condition then which parameter? if api change then which parameter? if change composition then which parameter? if old method not work out then whicj parameter? if additional one impurity added then which parameter of validation require to do on above each conditions? elaborate separately
discuss the diffrent types of chromatography and their uses. In not more than 10 papers oc not less than 8 papers
How to determine water content of bis tetrazole amine mono ammonium salt
Which parameters require to do in tech transfer?
why does used dry caffien in HPLC calibration?
Define dipole moment?
what is the disso medium for tropsiun chloride
