Answer / aroh shrivastava

Depending on the source, the protein has to be brought into
solution by breaking the tissue or cells containing it.
There are several methods to achieve this: Repeated
freezing and thawing, sonication, homogenization by high
pressure, clarification via cellulose-based depth filters,
or permeabilization by organic solvents. The method of
choice depends on how fragile the protein is and how sturdy
the cells are. After this extraction process soluble
proteins will be in the solvent, and can be separated from
cell membranes, DNA etc. by centrifugation. The extraction
process also extracts proteases, which will start digesting
the proteins in the solution. If the protein is sensitive
to proteolysis, it is usually desirable to proceed quickly,
and keep the extract cooled, to slow down proteolysis

Answer / guest

ZEOLITE METGHOD

NATURAL GAS ENGINEERING - QUESTION 26.2 : (a) The Hyperion sewage plant in Los Angeles burns 8 million cubic feet of natural gas per day to generate power in United States of America. If 1 metre = 3.28084 feet, then how many cubic metres of such gas is burnt per hour? (b) A reservoir of natural gas produces 50 mole % methane and 50 mole % ethane. At zero degree Celsius and one atmosphere, the density of methane gas is 0.716 g / L and the density of ethane gas is 1.3562 mg / (cubic cm). The molar mass of methane is 16.04 g / mol and molar mass of ethane is 30.07 g / mol. (i) Find the mass % of methane and ethane in the natural gas. (ii) Find the average density of the natural gas mixture in the reservoir at zero degree Celsius and one atmosphere, by assuming that the gases are ideal where final volume of the gas mixture is the sum of volume of the individual gases at constant temperature and pressure. (iii) Find the average density of the natural gas mixture in the reservoir at zero degree Celsius and one atmosphere, by assuming that the final mass of the gas mixture is the sum of mass of the individual gases. Assume the gases are ideal where mole % = volume % at constant pressure and temperature.

1 Answers  

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We have some pieces of metals that have been 'powder coated', how does that work?

0 Answers  

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How to determine the particle size distribution for a given bulk solid?

3 Answers  

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Explain how can one determine if a particular solid can be fluidized as in a fluidized bed?

0 Answers  

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Question 85 - (a) Three genes, I, J and K are available. All these genes are linked with respect to one another. If the percent recombination between I and J is 8 %, that between J and K is 10 %, and that between K and I is 18 %, what is the order of the gene? (b) Twenty six genes, a, b, c, d, e, f, ... x, y and z are available. All these genes are linked with respect to one another. If the percent recombination between a and b is 3 %, between b and c is 3 %, between c and d is 3 %, ... between w and x is 3 %, between x and y is 3 %, between y and z is 3 %, then what is the percentage recombination between b and y?

1 Answers  

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Is there a difference in mtd (mean temperature difference) between 'e' and 'j' (divided flow) type shell and tube heat exchangers?

0 Answers  

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PROCESS CONTROL - EXAMPLE 6.3 : The differential equation is 3 dy / dt + 2y = 1 with y(0) = 1. (a) The Laplace transformation, L for given terms are : L (dy / dt) = sY(s) - y(0), L(y) = Y(s), L(1) = 1 / s. Use such transformation to find Y(s). (b) The initial value theorem states that : When t approaches 0 for a function of y(t), it is equal to a function of sY(s) when s approaches infinity. Use the initial value theorem as a check to the answer found in part (a).

1 Answers  

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what is the main components of air

3 Answers   ONGC,

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QUANTUM CHEMISTRY AND CHEMICAL ENGINEERING - EXAMPLE 31.1 : As an approximation, let v = Zc / 137 where v is the radial velocity for 1 s electron of an element, c is the speed of light, Z is the atomic number. For gold with Z = 79, find the radial velocity of its 1 s electron, in term of c and percentage of the speed of light. (b) As an approximation, let A x A = 1 - Z x Z / 18769 where A is the ratio of the relativistic and non-relativistic Bohr radius. Find the value of A.

1 Answers  

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In DM plant, If pH in anion outlet is 7.2 and Mixed bed pH is 6.2, wat could be the reason.

8 Answers   Cipla, Jindal,

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ACCOUNTING AND FINANCIAL ENGINEERING - EXAMPLE 34.12 : You, as an engineer, buy a piece of biochemical instrument to start a servicing business. The instrument has a useful life of 3 years, that costs $60k. The instrumental business generates $100k per year. The labor costs $50k per year. The $60k new instrument depreciates in its value evenly up to 3 years, thereafter the old instrument needs to be replaced by another piece of new instrument. (i) In the first year of this engineering business : (a) what is its starting cash? (b) calculate the depreciation value of the instrument. (c) find the operating profit generated. (d) what is the capital expense of the business? (e) calculate the cash from operation. (f) calculate the ending cash. (ii) Based on the income statement of the business in the second year : (a) find the capital expense of the business. (b) what is its starting cash? (c) calculate the depreciation value of the instrument. (d) find the operating profit generated. (e) calculate the cash from operation. (f) calculate the ending cash.

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Question 79 - (a) The American Petroleum Institute gravity, or API gravity, is a measure of how heavy or light a petroleum liquid is compared to water. Let SG = specific gravity of petroleum liquid, and V = barrels of crude oil per metric ton. Given the formula for API gravity = 141.5 / SG - 131.5 and V = (API gravity + 131.5) / (141.5 x 0.159), find the relationship of SG as a function of V. (b) An oil barrel is about 159 litres. If a cylinder with diameter d = 50 cm and height h = 50 cm is used to contain the oil, find the volume V of the cylinder in the unit of oil barrel by using the formula V = 3.142 x d x h x d / 4. (c) First reference : 1 cubic metre = 6.2898 oil barrels. Second reference : 1 cubic metre = 6.37 oil barrels. What are the 2 factors that cause the difference in such reference data?

1 Answers  

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