Answer / aroh shrivastava

Depending on the source, the protein has to be brought into
solution by breaking the tissue or cells containing it.
There are several methods to achieve this: Repeated
freezing and thawing, sonication, homogenization by high
pressure, clarification via cellulose-based depth filters,
or permeabilization by organic solvents. The method of
choice depends on how fragile the protein is and how sturdy
the cells are. After this extraction process soluble
proteins will be in the solvent, and can be separated from
cell membranes, DNA etc. by centrifugation. The extraction
process also extracts proteases, which will start digesting
the proteins in the solution. If the protein is sensitive
to proteolysis, it is usually desirable to proceed quickly,
and keep the extract cooled, to slow down proteolysis

Answer / guest

ZEOLITE METGHOD

What are the some common problems associated with dense phase pneumatic conveying?

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COMPUTER PROGRAMMING FOR ENGINEERS - EXAMPLE 17.2 : There are 5 simultaneous equations with 5 unknowns as follow : 16 V - 4 X - Z = 36, 4 X - 4 W - Y + Z = 3, 8 V + 4 W - X + Y = 10, X - W = 1, V + W = 0. Find the values of V, W, X, Y and Z accurately within 15 minutes. State the computer program that you use to get the answers.

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PROCESS DESIGN - EXAMPLE 21.2 : The names of the flow streams could be represented by : H1 for first hot stream, H2 for second hot stream, C1 for first cold stream, C2 for second cold stream. Data of supply temperature Ts in degree Celsius : 150 for H1, 170 for H2, 30 for C1, 30 for C2. Data of target temperature Tt in degree Celsius : 50 for H1, 169 for H2, 150 for C1, 40 for C2. Data of heat capacity Cp in kW / degree Celsius : 3 for H1, 360 for H2, 3 for C1, 30 for C2. (a) Find the enthalpy changes, dH for all streams of flow H1, H2, C1 and C2 in the unit of kW. Take note of the formula dH = (Cp) (Tt - Ts). (b) Match the hot streams H1 and H2 with the suitable cold streams C1 and C2 to achieve the maximum energy efficiency.

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How does a chemical get ignited without external heat source on reaching its auto ignition temperature

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Hi this is aparna.............can any one send drdo question papers on my email id-aparna_patna08@yahoo.com Thanks in advance

2 Answers   DRDO,

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ENGINEERING MECHANIC - EXAMPLE 15.2 : A cantilever beam of length L = 3 m carries a uniformly distributed load (UDL) of W = 20 N / m throughout the length. Calculate the bending moment, BM of the beam near the fixed end. What is the shear force, SF at this point? Let SF = -WL, BM = -LWL/2.

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ACCOUNTING AND FINANCIAL ENGINEERING - EXAMPLE 34.3 : (a) In the M / M / 1 queue that happens with randomness, let State 0 = the queue and server are empty, State 1 = the server is in use and the queue is empty, State 2 = the server is in use and 1 is in the queue, State 3 = the server is in use and 2 in the queue. Let P (0) = probability of State 0, P (1) = probability of State 1, P (2) = probability of State 2, P (3) = probability of State 3 and so on. If c = constant, P (1) = c P (0), P (2) = c [ c P (0) ], P (3) = c { c [ c P (0) ] }, write an equation that involves P (N), P (N + 1) and c. (b) Let L = market price of risk, r = riskless rate, m = expected return, s = volatility. Given that L = (m - r) / s related to oil prices, expected return = 12 %, s = 20 %, riskless rate = 8 %, calculate the market price of risk.

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According to Shockley equation, the I – V characteristic of a diode is approximated by I = IS [ exp (nVD / VT) – 1 ]. For silicon, let the reverse bias saturation current IS as 0.000000000001. If n is ideality factor with value of 1.5, VT as thermal voltage drop of 0.026 V at room temperature, what is the value of current I that passes through the silicon diode in the heater of evaporator when the forward voltage drop VD = 0.026 V? Please take note that exp is the exponential function with e(1) = 2.718, e(2) = 7.389.

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Question 35 – A mixture consists of benzene (B), toluene (T) and xylene (X). At a temperature of 353 K, the data of vapor pressures : B : 754.12, T : 289.71, X : 91.19. Unit is mm Hg. The pressure P is 0.5 atm. The value of k for each substance is k = (vapor pressure) / P. (a) Calculate k for B, T and X. Let L / V = 0.65. (b) By using the equation V = F / [ (L / V) + 1 ], find the value of V when F = 100, then what is the value of L?

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Can any one send me the sample test papers of IOCL entry exam to be held on 6th july 2008. Also plz send the interview questions that cud probably be asked........i wud be highly obliged if u can send a few test paers and other stuff @ inderjeetd@gmail.com.........Inderpreet

0 Answers   IOCL,

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What are some factors to consider when trying choosing between a dry screw compressor and an oil-flooded screw compressor?

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on which website can i get previous GATE question papers?

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