Answer / aroh shrivastava

Depending on the source, the protein has to be brought into
solution by breaking the tissue or cells containing it.
There are several methods to achieve this: Repeated
freezing and thawing, sonication, homogenization by high
pressure, clarification via cellulose-based depth filters,
or permeabilization by organic solvents. The method of
choice depends on how fragile the protein is and how sturdy
the cells are. After this extraction process soluble
proteins will be in the solvent, and can be separated from
cell membranes, DNA etc. by centrifugation. The extraction
process also extracts proteases, which will start digesting
the proteins in the solution. If the protein is sensitive
to proteolysis, it is usually desirable to proceed quickly,
and keep the extract cooled, to slow down proteolysis

Answer / guest

ZEOLITE METGHOD

Explain the different characteristics that can affect the flow of bulk solids and how?

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Explain how can you prevent bridging in a dilute phase pneumatic conveying system?

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QUANTUM CHEMISTRY AND CHEMICAL ENGINEERING - EXAMPLE 31.9 : When an algebraic product is defined on the space, the Lie bracket is the commutator [x,y] = xy - yx according to Lie algebra in mathematics. If [p,x] f(x) = px f(x) - xp f(x), p = -ih d / dx, find the value of [p,x] in term of i and h.

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ENGINEERING MATHEMATICS - EXAMPLE 8.2 : In the US (United States), one barrel is equal to 42 US gallons and equivalent to 0.15898 cubic metres. One British barrel is equal to 36 Imperial gallons and equivalent to 0.163659 cubic metres. If an oil refinery in England sells 42 Imperial gallons of crude oil to a company in US, how much US gallons of crude oils has been sold?

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How to TDS removed found in lab apparatus.

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Experiment constitutes 1000 ml of 10 ppm dye solution and 0.2 g catalyst mixed in beaker. How to calculate the initial of concentration?

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how to decide reflux ratio & its calculation

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Question 67 – In order to predict the wastewater production, the population number has to be understood. The population data is : 72000 (for year 1961 or P-1961), 85000 (for year 1971 or P-1971), 110500 (for year 1981 or P-1981). (a) Find the average population increase, or [ (P-1981 - P-1971) + (P-1971 - P-1961) ] / 2. (b) Find the average percentage population increase, or [ (P-1981 - P-1971) / P-1971 + (P-1971 - P-1961) / P-1961 ] / (2) X 100. (c) Find the incremental increase or P-1981 - 2 (P-1971) + P-1961. (d) Let Po = P-1981. After 2 decades or n = 2, the population is P-2001. By using arithmetical increase method, find P-2001 = Po + n (Answer for a). (e) By using incremental increase method, find P-2001 = (Answer of d) + n (n + 1) (Answer of c) / 2. (f) By using geometrical increase method, find P-2001 = Po [ 1 + (Answer of b) / 100 ] ^ n where ^ is power sign, or 1 ^ 2 = 1 x 1 = 1. (g) If the actual P-2001 = 184000, which method of estimation is more accurate, based on your answer in (d), (e) and (f)?

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What is the characteristic length for slab?

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Do you have recombinant protein expression experience? Explain?

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what is zeroth law ?

6 Answers   ISRO,

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Question 84 - In Mendelian genetics, yellow (Y) is dominant to green (y) and round (R) is dominant to wrinkled (r). (a) What is the probability P of Rr x Rr producing wrinkled seeds? (b) What is the probability P of Yy x yy producing green seeds? (c) What is the probability that RRYy x RrYy would produce RrYy?

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