Interested to Buy Any Domain ? << Click Here >> for more details...
Answer Posted / aroh shrivastava
Depending on the source, the protein has to be brought into
solution by breaking the tissue or cells containing it.
There are several methods to achieve this: Repeated
freezing and thawing, sonication, homogenization by high
pressure, clarification via cellulose-based depth filters,
or permeabilization by organic solvents. The method of
choice depends on how fragile the protein is and how sturdy
the cells are. After this extraction process soluble
proteins will be in the solvent, and can be separated from
cell membranes, DNA etc. by centrifugation. The extraction
process also extracts proteases, which will start digesting
the proteins in the solution. If the protein is sensitive
to proteolysis, it is usually desirable to proceed quickly,
and keep the extract cooled, to slow down proteolysis
| Is This Answer Correct ? | 14 Yes | 2 No |
Post New Answer View All Answers
Explain some good strategies for curing tube vibration in shell and tube exchangers?
What is a solvent?
X is a solid having a white colour at room temperature. It has a density about 2g/cc. Although it has melting point near 325 degree Celsius, its properties start degrading above 260 degree Celsius. The coefficient of friction is very low about 0.1. It has very good dielectric properties especially at higher radio frequencies. It has a very high bulk resistivity. It is chemically inert. It is also resistant to van der Waals force. It is hydrophobic as well as lipophobic. Creep or ‘Cold Flow’ has been observed in X.
Explain how can I evaluate the thermal relief requirements for double block-in of 98% sulfuric acid?
What is gibbs free energy?
Jspl question paper for freshers requirements chemical engineering diploma
What does the catalytic converter on an automobile really do?
Do you have any idea on recombinant protein expression?
Explain what is a good way to get started in doing a plant-wide steam consumption analysis?
Can large temperature differences in vaporizers cause operational problems?
Does home used kerosene which is available in blue color can change its color after keeping number of years (say 8-10 years)? If yes, then in which color will it turned to? Will it change to yellow color or transparent color? Please reply with suitable technical suggestion/approach. Its an important question which was asked. I need a very strong technical reply. Please help me.
Hi.. Me going to tke test for management trainee(technical) Post I am an chemical engineering student . Can u guide how to prepare for this test.Can u send me test paper pattern..?
Why is post-weld heat treatment sometimes necessary for welded vessels?
What are the types of load?
Is there any way to repair a valve that is passing leaking internally without taking our process offline?
