Question 89 - A hemocytometer is a device that is used for counting cells. In an engineering experiment, 100 microlitres of cell suspension is diluted with 50 microlitres of Trypan blue dye. Only death cells appear blue in color when stained with the dye. There are 57 cells detected in a hemocytometer, where 5.3 % of them appear blue when the chamber of the meter is placed under a microscope. Each square of a chamber can contain 0.0001 mL of liquid. (a) Calculate the number of viable cells. (b) The cells ocupied 5 squares. Calculate the average number of viable cells / square. (c) Calculate the dilution factor of the cell suspension by using the formula : Dilution = final volume / initial volume. (d) Calculate the concentration of viable cells / mL by using the formula : Concentration = (Average number of viable cells / square) x dilution x (square / volume).

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Is there a rule of thumb to estimate the footprint of a cooling tower during design phase?

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Why water is not directly added to sulphuric acid for dilution?

8 Answers  

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Any books are availble to read for industrial promotional officers exam.. Syllabus : PAPER-2 : Subject (Diploma Standard) Applied Science, Engineering and Management 1. MATHEMATICS 2. PHYSICS 3. CHEMISTRY 4. INDUSTRIAL MANAGEMENT 5. INDUSTRIAL ENGINEERING 6. COMPUTER APPLICATIONS & BASICS OF DOS, WINDOWS, UNIX, DB MANAGEMENT, DTP, CONCEPTS OF E-GOVERNANCE INCLUDING E-MAIL, E-COMMERCE, INTERNET, COMPUTER VIRUSES.

3 Answers   APPSC,

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can anybody give me syallbus of chemical engineering for section engineer in railway.

0 Answers   CCIL,

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i badly need a chemical process details for making a project...plz help me out......i m a btech final yr chemical engg student in a govt univ,and we have to make a detailed project on any chemical process with contents like...intro,kinetics , material balance,thermodynamics,process layout,process design,physical conditions,plant location,cost estimation etc.............if any one could help me i will never forget hi thankless work and always try to help u in whatever u ask.............

1 Answers   HPCL,

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Explain the functioning of a three-way catalytic converter.

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sir,please give me some exam paper for referance. Thanks & Regards,

0 Answers   ONGC,

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How can hexavalent chromium be removed from aqueous waste streams?

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GENETIC ENGINEERING - EXAMPLE 27.4 : According to Hardy-Weinberg Equation, p x p + 2 x p x q + q x q = 1 where p = dominant allele frequency and q = recessive allele frequency. Let p + q = 1. Fraction of population has 2 copies of the p gene = p x p. Fraction of population has 2 copies of the q gene = q x q. Fraction of population has a copy of p gene and a copy of q gene = 2 x p x q. In a small town, the allele frequency is q = 0.2 for a recessive gene, the delta-32 mutation, that gives human protection from HIV infection. (a) Find the allele frequency of a dominant gene, p. (b) What percent of the population has at least a copy of the gene that cause the population either immune to HIV or less susceptible to the disease?

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ACCOUNTING AND FINANCIAL ENGINEERING - EXAMPLE 34.7 : An engineering investor purchases a $1000 bond that matures in 25 years. The coupon rate is 8 %. The purchase price is at 95 or 95 % of the par bond value. (a) What is the purchase price of the bond? (b) How much discount is enjoyed by the investor on average each year? (c) Find the income generated from coupon rate each year. (d) Find the overall interet rate or Yield to Maturity (YTM) by using the formula : YTM = [ Answer in (c) + Answer in (b) ] x 200 / [ par bond value + Answer in (a) ].

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MY generator water cooler inlet line height - 11 mtr inlet water line height - 7 mtr water come into the line from overhead tank, O/H tank height - 20 mtr the first tapping in the inlet line is 2" line which down to 4 mtr level plateform., second tapping in inlet water line goes to a pump 6" line installed at 4 mtr level plateform, pump capacity 200m3/hr, head- 30 mtr, hp-40. the outlet of pump of 6" water line connected to a header of 6 mtr long of 6" line, on this header the six tapping are connected, 4 nos. 3" line goes to 11 mtr height water cooler the pressure of the line is 0.15 to 0.25 kg/cm2, 2" and 4" pipe line connected to a cooler at 4 mtrlevel plateform. my question is, at 11 mtr height level the quantity of water and flow is not sufficient for my cooler, the temp. of cooler is rises much more. you are requested to suggest the modification of pipe line/ pump for take more water flow and pressure (desired pressure is 1 kg/cm2)

0 Answers   Simbhaoli Sugars,

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