Question 89 - A hemocytometer is a device that is used for counting cells. In an engineering experiment, 100 microlitres of cell suspension is diluted with 50 microlitres of Trypan blue dye. Only death cells appear blue in color when stained with the dye. There are 57 cells detected in a hemocytometer, where 5.3 % of them appear blue when the chamber of the meter is placed under a microscope. Each square of a chamber can contain 0.0001 mL of liquid. (a) Calculate the number of viable cells. (b) The cells ocupied 5 squares. Calculate the average number of viable cells / square. (c) Calculate the dilution factor of the cell suspension by using the formula : Dilution = final volume / initial volume. (d) Calculate the concentration of viable cells / mL by using the formula : Concentration = (Average number of viable cells / square) x dilution x (square / volume).
Answer 89 - (a) Number of death cells = Total cells x % of death cells / 100 = 57 x 5.3 / 100 = 3.021 rounded to 3 cells. Number of viable cells = Total cells - number of death cells = 57 - 3 = 54 cells. (b) Average number of viable cells / square = 54 / 5 = 10.8 cells / square. (c) Dilution = final volume / initial volume = (100 + 50) microlitres / 100 microlitres = 1.5. (d) Concentration = (Average number of viable cells / square) x dilution x (square / volume) = 10.8 cells x 1.5 x (1 / 0.0001 mL) = 162000 cells / mL. The answer is given by Kang Chuen Tat; PO Box 6263, Dandenong, Victoria VIC 3175, Australia; SMS +61405421706; chuentat@hotmail.com; http://kangchuentat.wordpress.com.
Is This Answer Correct ? | 0 Yes | 0 No |
On what basis are materials to be crushed evaluated apart from size?
A cantilever beam of length L = 3 m carries a uniformly distributed load (UDL) of W = 20 N / m throughout the length. Calculate the bending moment, BM of the beam near the fixed end. What is the shear force, SF at this point? Let SF = -WL, BM = -LWL/2.
ENGINEERING MATHEMATICS - EXAMPLE 8.4 : Let 1 ^ 1 = 1, 2 ^ 2 = 4, 3 ^ 3 = 27. By using the Excel computer programming - either by Solver or Goal Seek, find the value of v for the Van der Waals equation (P - a / v ^2) (v - b) = RT where a = 18.82, b = 0.1193, P = 2, R = 0.082, T = 5000 for benzene. Describe briefly how to use Solver and Goal Seek in Excel program of computer to find the solution quickly.
where can i get previous question papers of GATE in chemical engineering
how to calculate overall heat transfer coefficient in insulated pipeline? Please give a answer with formula
how interstage pressure of multistage compressor is calculated?
What is the best way to handle bend or turns in slurry piping systems?
plz send the interview questions in the written test asked by hindustan petroleum corporation ltd.Iam a b>tech in chemical engineering.My written test is on feb 2008.urgent plz.send the questions to avazithraja@rediffmail.com
Question 41 - According to Raoult's law, the pressure when vapor is completely condensed, P = x(1) P(1) + x(2) P(2) ... + x(n) P(n) when x(1), x(2) ... x(n) are the mole fractions of component 1, 2 ... n and P(1), P(2) ... P(n) are the vapor pressures of component 1, 2 ... n. A vapor at 74 degree Celsius containing 70 mole % water and 30 mole % ethanol is to be completely condensed. At the temperature of 74 degree Celsius vapor pressure is 0.38 atm for water and 0.97 atm for ethanol. What is the minimum pressure the compressor must be operated?
ACCOUNTING AND FINANCIAL ENGINEERING - EXAMPLE 34.15 : Based on Time Discounting of Money, let y = x / (1 + r) ^ n, where ^ = power, r = interest rate for the period in decimal value, n = number of periods, y = current value, x = future value. According to Present Value Addition Rule, the present value of a set of future payments is equal to sum for present value of each of the payments. In an engineering business, 3 future payments are to be made : $100 after 1 year; $500 after 2 years; $200 after 3 years. (a) Form an equation of y as a function of r. (b) Find the value of y in 5 decimal places if r = 8 %.
What is the order of a chemical reaction, , if the rate of formation of 'C', increases by a factor of 2.82 on doubling the concentration of 'A' and increases by a factor of 9 on trebling the concentration of 'B'?
Are there any general rules that should be considered when designing a slurry piping system?