Question 89 - A hemocytometer is a device that is used for counting cells. In an engineering experiment, 100 microlitres of cell suspension is diluted with 50 microlitres of Trypan blue dye. Only death cells appear blue in color when stained with the dye. There are 57 cells detected in a hemocytometer, where 5.3 % of them appear blue when the chamber of the meter is placed under a microscope. Each square of a chamber can contain 0.0001 mL of liquid. (a) Calculate the number of viable cells. (b) The cells ocupied 5 squares. Calculate the average number of viable cells / square. (c) Calculate the dilution factor of the cell suspension by using the formula : Dilution = final volume / initial volume. (d) Calculate the concentration of viable cells / mL by using the formula : Concentration = (Average number of viable cells / square) x dilution x (square / volume).
Answer 89 - (a) Number of death cells = Total cells x % of death cells / 100 = 57 x 5.3 / 100 = 3.021 rounded to 3 cells. Number of viable cells = Total cells - number of death cells = 57 - 3 = 54 cells. (b) Average number of viable cells / square = 54 / 5 = 10.8 cells / square. (c) Dilution = final volume / initial volume = (100 + 50) microlitres / 100 microlitres = 1.5. (d) Concentration = (Average number of viable cells / square) x dilution x (square / volume) = 10.8 cells x 1.5 x (1 / 0.0001 mL) = 162000 cells / mL. The answer is given by Kang Chuen Tat; PO Box 6263, Dandenong, Victoria VIC 3175, Australia; SMS +61405421706; chuentat@hotmail.com; http://kangchuentat.wordpress.com.
| Is This Answer Correct ? | 0 Yes | 0 No |
UNIT OPERATION - EXAMPLE 9.4 : Acetone and ethanol are separated using a distillation column with a partial condenser and partial reboiler. An equimolar, sub-cooled liquid feed enters at 100 kmol / hr and condenses 1 mole of vapor for every 6 moles of feed. The separation requires a distillate vapor that is 95 mol % acetone and bottoms liquid that is 5 mol % acetone. The reflux is returned from the condenser to the column as a saturated liquid and the operation is run at (L / V) = 1.4 * (L / V) min. Assume constant overflow conditions. (a) Feed operating line is y = [ q / (q - 1) ] x - z / (q - 1) where z = 0.5 for equimolar liquid mixture of 2 components, q = (L'- L) / F where L' = L + F + (F / 6) for condensation of 1 mole of vapor / 6 moles of feed. What is y = f(x)? (b) The rectifying operating line is y = (L / V) x + (D / V) (xd) where (L / V) min goes through the points A (0.95, 0.95) and B (0.53, 0.69), V = L + D. What is y = f(x)? Let xd = 0.95. (L / V) min is the slope of the 2 points A and B.
How to estimate the efficiency of a pump?
What is the principles of crayogenic expansion turbin
what is mean by reflux ratio?
What are some factors to consider when trying choosing between a dry screw compressor and an oil-flooded screw compressor?
ENGINEERING MECHANIC - EXAMPLE 15.2 : A cantilever beam of length L = 3 m carries a uniformly distributed load (UDL) of W = 20 N / m throughout the length. Calculate the bending moment, BM of the beam near the fixed end. What is the shear force, SF at this point? Let SF = -WL, BM = -LWL/2.
Question 50 - An aqueous solution with 2.5 g of a protein dissolved in 600 cubic centimeters of a solution at 20 degree Celsius was placed in a container that has a water-permeable membrane. Water permeated through the membrane until the h - level of the solution was 0.9 cm above the pure water. (a) Calculate the absolute temperature of the solution, T in Kelvin, where T (Kelvin) = T (degree Celsius) + 273.15. (b) Calculate the osmotic pressure, P of the solution by using the formula P = hrg where h is level of the solution, r is density of water with 1000 kg per cubic meter, g = 9.81 N / kg as gravitational acceleration. (c) Calculate the concentration of the protein solution, C in kg / cubic meter. (d) Calculate the molecular weight of the protein, (MW) = CRT / P where R = 8.314 Pa cubic meter / (mol K) as ideal gas constant.
pls send me model paper of NPCIL for executive trainee. and syllabus of executive trainee 2015.
can anybody give me syallbus of chemical engineering for section engineer in railway.
Question 66 – The drag coefficient Cd = 0.05 and lift coefficient Cl = 0.4 for a levelled flow aircraft are measured. The velocity of the aircraft is v = 150 ft / s with its weight W = 2677.5 pound-force. (a) Find the value of the lift of the aircraft, L, when it is also its weight. (b) The drag of the aircraft, D = Cd M, L = Cl M. Find the value of D. (c) The power required is P = Dv. If 1 pound-force x (ft / s) = 1.356 W, find the value of P in the unit of Watt or W.
How are plate heat exchangers used in an ammonia refrigeration system?
Any books are availble to read for industrial promotional officers exam.. Syllabus : PAPER-2 : Subject (Diploma Standard) Applied Science, Engineering and Management 1. MATHEMATICS 2. PHYSICS 3. CHEMISTRY 4. INDUSTRIAL MANAGEMENT 5. INDUSTRIAL ENGINEERING 6. COMPUTER APPLICATIONS & BASICS OF DOS, WINDOWS, UNIX, DB MANAGEMENT, DTP, CONCEPTS OF E-GOVERNANCE INCLUDING E-MAIL, E-COMMERCE, INTERNET, COMPUTER VIRUSES.
Civil Engineering (5086)
Mechanical Engineering (4453)
Electrical Engineering (16638)
Electronics Communications (3918)
Chemical Engineering (1095)
Aeronautical Engineering (239)
Bio Engineering (96)
Metallurgy (361)
Industrial Engineering (259)
Instrumentation (3014)
Automobile Engineering (332)
Mechatronics Engineering (97)
Marine Engineering (124)
Power Plant Engineering (172)
Textile Engineering (575)
Production Engineering (25)
Satellite Systems Engineering (106)
Engineering AllOther (1379)