how to correct peak when peak comes broader in shape?
How to do calibration of HPLC?
Answers were Sorted based on User's Feedback
Answer / satish
if peak is broad then use dilute solution of triethylamine to sharpen the peak
| Is This Answer Correct ? | 5 Yes | 1 No |
Answer / chanti1984
1. ENHANCE THE COLUMN OVEN TEMPARATURE, REDUCE THE SAMPLE
LOAD N COLUMN AND CHANGE THE DILUENT
2. URACIL/CAFFIENE SOLUTION ARE USING FOR CALIBRATION OF
HPLC WITH THEIR RESPECTIVE WAVE LENGTH
| Is This Answer Correct ? | 1 Yes | 2 No |
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2. Two grams of Benzoic acid are dissolved in 200 ml of water and extracted with 200 ml of diethyl ether. The distribution coefficient of benzoic acid is 100, and its dissociation constant is 6.5 10-5. Calculate the distribution ratio (D) of benzoic acid at pH 2, 5, and 6. 3. Calculate D at pH 2 to 10 (1 unit apart) in the above problem, and plot D versus pH.