how to correct peak when peak comes broader in shape?
How to do calibration of HPLC?

Answers were Sorted based on User's Feedback



how to correct peak when peak comes broader in shape? How to do calibration of HPLC? ..

Answer / satish

if peak is broad then use dilute solution of triethylamine to sharpen the peak

Is This Answer Correct ?    5 Yes 1 No

how to correct peak when peak comes broader in shape? How to do calibration of HPLC? ..

Answer / chanti1984

1. ENHANCE THE COLUMN OVEN TEMPARATURE, REDUCE THE SAMPLE
LOAD N COLUMN AND CHANGE THE DILUENT
2. URACIL/CAFFIENE SOLUTION ARE USING FOR CALIBRATION OF
HPLC WITH THEIR RESPECTIVE WAVE LENGTH

Is This Answer Correct ?    1 Yes 2 No

Post New Answer

More Analytical Chemistry Interview Questions

what is mean by theoritical plates(not formula) ?

4 Answers   Micro Labs,


why we use polystyrene film for ftir calibration?

4 Answers   Rakshit Drugs,


Why we more noise in PDA detector compared to UV detector?

1 Answers   Aurobindo,


why kf factor coming 0.5

5 Answers   Alkem Labs,


How is the Flame test performed?

4 Answers   Lupin,






what is the disso medium for tropsiun chloride

0 Answers  


why should we perform dissolution PVT calibration only by UV spectrometer, not by HPLC ?

0 Answers  


what are the different methods of analysing related substances test,what are LOD AND LOQ .HOW TO REPORT IMPURITIES WHEN LOD AND LOQ LIMITS R SPECIFIED

3 Answers   Dr Reddys,


Why sulfuric acid is used in sulphated ash / residue on ignition test.

9 Answers   CS Pharmaceuticals, Dr Reddys, GVK, HPLC, Micro Labs, Samrudh, Watson Pharmaceuticals, Wings Customer Care,


Why we are use 0.005M Sulphuric acid, in control of absorbance performance test UV Spectrophotometer calibration

2 Answers   Cipla, Reddy Labs,


how to prepare 5normality of hcl

2 Answers  


When we r doing processing method for sst in Empower software by HPLC, What value has to give in void volume and as per which pharmacopia!

5 Answers   Micro Labs, Mylan,


Categories
  • Organic Chemistry Interview Questions Organic Chemistry (342)
  • Inorganic Chemistry Interview Questions Inorganic Chemistry (123)
  • Analytical Chemistry Interview Questions Analytical Chemistry (1387)
  • Physical Chemistry Interview Questions Physical Chemistry (62)
  • General Chemistry Interview Questions General Chemistry (478)
  • Chemistry AllOther Interview Questions Chemistry AllOther (190)