how to correct peak when peak comes broader in shape? How to do calibration of

how to correct peak when peak comes broader in shape?
How to do calibration of HPLC?

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how to correct peak when peak comes broader in shape? How to do calibration of HPLC? ..

Answer / satish

if peak is broad then use dilute solution of triethylamine to sharpen the peak

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how to correct peak when peak comes broader in shape? How to do calibration of HPLC? ..

Answer / chanti1984

1. ENHANCE THE COLUMN OVEN TEMPARATURE, REDUCE THE SAMPLE
LOAD N COLUMN AND CHANGE THE DILUENT
2. URACIL/CAFFIENE SOLUTION ARE USING FOR CALIBRATION OF
HPLC WITH THEIR RESPECTIVE WAVE LENGTH

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