why in hplc peaks are in positive side(it means upside) not in
negative side?
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Answer / sagar mhaske
BEZ we are taking reading in mv,not in transmittance so all peak in hplc on positive side..
if we are taking reading in transmittance then automatically goes negative side.
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Answer / rajeev sharma
Polarity of mobile phase less to diluent so negative peak generated
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