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Question 90 - In the calculation of the growth of bacteria, absorbance, A in spectrophotometry is used. According to Beer-Lambert Law, A = e x l x c where A is the absorbance of the solution (no unit), l is the distance of light travels through the solution (in cm), e is the molar absorptivity or the molar extinction coefficient [ in L / (mol.cm) ]. For a particular solute and fixed path length : As / Ao = Cs / Co where Ao is the observed signal for a known concentration Co, and As is the observed signal for a sample concentration Cs. (a) For a cell concentration of 560 cells / mL, a spectrophotometre gives an absorbance reading of 1.0. A mixture of concentration 3600000 cells / mL can be diluted in several operations, with each operation having a dilution of 1:20. How many dilutions should be made so that the concentration of this mixture can be calculated within a range of A = 0.0 to 1.0. (b) In another experiment, a sample tube of 1 cm in width is used. Let A = 0.06 and e = 0.0012 ml / (cell.cm). Find the cell concentration of the sample.

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What are flameless oxidizers?

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Why an electromagnetic flow meter cannot be used for gases, steam and oil flow measurements?

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Question 47 - In a cylinder with a hollow, let a is outside radius and b is the inside radius. In a steady state temperature distribution with no heat generation, the differential equation is (d / dr) (r dT / dr) = 0 where r is for radius and T is for temperature. (a) Integrate the heat equation above into T(r) in term of r. (b) At r = a, T = c; at r = b, T = d. Find the heat equation of T(r) in term of r, a, b, c, d.

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Dielectric materials in static fields

0 Answers   HCL,

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What are some guidelines for designing for liquid and gas velocities in process plant piping?

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In Galvonised iron and stainsteel pipes, in which pipe friction losses will more and why ?

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How to size the cooling tower sump / basin for any given circulation rate? What is the guideline?

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Is there a rule of thumb to estimate the footprint of a cooling tower during design phase?

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Please describe tar, asphalt and bitumine

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all subject of chemical engg

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When specifying a cooling tower, should I look up historic wet bulb temperatures for my area or should I take measurements?

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