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In method developement or in any analysis if we use small
length column for analysis what happen then ? why ?
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Answer / kishore
retention times, analysis run times, back pressure will
reduced.
May mix closely eluting compounds
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Answer / dharmesh bhatt
the question is not very specific, however, if one can use the shorter column for method development, they can optimize the method without sacrificing peak resolution. the theoretical plate and pressure will depend on the type of column & instrument one can select for analysis.
if the shorter column is used for analysis, it will shorten the analysis time and the resolution will sacrifice but again this will depend on the column and instrument.
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Resolution and theoretical plate count will also decrease
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