Answer / ch.h.n.prasad reddy

Peak purity in HPLC is nothing but SPECTRUL PURITY not
Chemical purity. In HPLC Each peak is combination of many
data points. Each data point is having SPECTRUM. In peak
purity calculation each data point SPECTRUM is compared
with SPECTRUM of Peak apex Data point. In this we can
compare deferent region data points with Peak apex Data
point SPECTRUM. At that time we consider Base line noise,
Solvent noise etc.... we can select wave length range
also....
Always Purity angle is less than threshold then only Peak
Purity can pass.
Best Example: Isomers
Both isomers can show same spectrum but chemically they
are different. In this case if we get single peak also our
Peak Purity can Pass because our Peak apex Data point
SPECTRUM and deferent regions in that Peak data points
SPECTRUM is SAME.

Thanks
CH.H.N.PRASAD REDDY
RESEARCH SCIENTIST
prasadreddy19@gmail.com

Answer / saran

In PDA Detector, Purity angle value is less than the threshold

Answer / umesh patel

IT IS DEFINED AS PURITY OF A PERTICULAR PEAK THAT IT IS NOT
COELUTED WITH OTHER IMPURITIES

PURITY=RATIO OF ABSORBANCE B/W THE ROOT OF UP SLOPE AND
APEX/RATIO OF ABSORBANCE B/W THE ROOT OF DOWN SLOPE AND APEX

IF THE PURITY=1, PURE PEAK

Answer / ramakrishna

to identify whether the particular peak is single or
combination of peaks.
if angle is less than threshold the peak is pure.
if angle is more than threshold the peak is impure.

Answer / g.venkata suresh

Peak purity is nothing but purity angel should be less than purity threshould

Purity angel is a measure of the spectrul hetrogeneity of the peak based on the spectrum overall the peak using spectrum constract angel.

Spectral constract angel is defined as the shape difference between two spectra.

Purity threshould is a minimum purity of the reference standard in the formulation.

Answer / rashid ansari

Dilute o.1 ml phosphoric acid to 100 ml with water

Answer / kuchela rao athota

spectra are used to calculate the purity level of the peak,
or to construct the similar curves.
Similarity/Threshold
Ln(Similarity)
Similairty/Threshold ratio

Answer / jilani

Peak purity means 100% purity of drug. SPECTRUL PURITY not
Chemical purity.

Answer / anil kumar

Peak purity we have to check through the PDA detector. It
should be NLT99%.Peak purity we can check in 3D from uv to
visible range.

Answer / ganesh bhavar

peak purity means 100% pure peak of drug

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