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Answer Posted / ch.h.n.prasad reddy
Peak purity in HPLC is nothing but SPECTRUL PURITY not
Chemical purity. In HPLC Each peak is combination of many
data points. Each data point is having SPECTRUM. In peak
purity calculation each data point SPECTRUM is compared
with SPECTRUM of Peak apex Data point. In this we can
compare deferent region data points with Peak apex Data
point SPECTRUM. At that time we consider Base line noise,
Solvent noise etc.... we can select wave length range
also....
Always Purity angle is less than threshold then only Peak
Purity can pass.
Best Example: Isomers
Both isomers can show same spectrum but chemically they
are different. In this case if we get single peak also our
Peak Purity can Pass because our Peak apex Data point
SPECTRUM and deferent regions in that Peak data points
SPECTRUM is SAME.
Thanks
CH.H.N.PRASAD REDDY
RESEARCH SCIENTIST
prasadreddy19@gmail.com
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