Interview Questions Answers in Analytical Chemistry

What about karl fischer reaction mechanism ?

Answer

When sample is introduced in to the titration cell and after dissolved,reagent is released due to induction of electrical current. The amount of current required to covert water is the determinant of amount of moisture.
Thanks,
B.Gangatharan,
Caplin point,
Pondicherry(now puducherry)

Is This Answer Correct ?

0 Yes

0 No

Question { Sequent, 4829 }

Why we perform the ir analysis?

Answer

IR analysis is important test to identify compounds by determining functional groups.it is one of the important identification test in pharma.companies.
Thanks,
Gangatharan.B
Caplin point.

Is This Answer Correct ?

1 Yes

0 No

Question { Nestle, 4005 }

What is FFA, PV, and PAV value in oil.

Answer

FFA -means Free fatty acid value in oil
PV-peroxide value in oil
PAV-Para Anisidine Value in oil

Is This Answer Correct ?

2 Yes

0 No

Question { 2578 }

When recording an IR spectrum in the lab, we take no special precautions to account for the presence of oxygen, nitrogen, or argon in the air. What are the consequences of this?

Answer

Argon gas consists of individual atoms which can not undergo molecular vibration .So argon is IR inactive.oxygen & Nitrogen is having dipole moment zero during vibration.so Oxygen&Nitrogen is IR inactive.hence it is not necessary to consider this gases during IR analysis.
Thanks,
B.Gangatharan,
Caplin Point,
Puducherry.

Is This Answer Correct ?

2 Yes

0 No

Question { 4615 }

What is difference between" LOD" AND "VACCUME LOD"

Answer

Vacuum lod is used to minimise oxidation during drying and for reducing drying time also.it is used for low melting range materials.vacuum during is generally used for hygroscopic and heat sensitive materials.
Thanks,
B.gangatharan,
Caplin point,
Puducherry.

Is This Answer Correct ?

9 Yes

0 No

Question { 9809 }

Difference between standard operating procedure and standard test procedures in pharmaceutical industry

Answer

SOP is classified as 3 types.
1.general sop. 2.system sop 3.operation sop ,in the above said,operation sop deals with how to operate & calibrate the instrument.
STP is describing the standard method of analysis
Thanks,
Gangatharan.B

Is This Answer Correct ?

7 Yes

0 No

Question { 2416 }

How to define samples weight in moisture content

Answer

According to USP general chapter<921>where the specimen under test is capsule,use a portion of mixed contents of not fewer than 4 capsules.when specimen under test is tablets,use powder from not fewer than 4tablets ground to fine powder in an atmosphere of temperature and RH known not to influence the results.
Thanks,
B.Gangatharan,
Caplin point,
Pondicherry.

Is This Answer Correct ?

2 Yes

0 No

Question { Aurobindo, 3769 }

What is differences between the Instrument and Equipment

Answer

We can not get results directly from equipment.but we can get results directly from instrument.
Thanks,,
B.gangatharan
Caplin point.

Is This Answer Correct ?

22 Yes

0 No

Question { Sanmar, 3732 }

What is UV cut off?

Answer

UV cut off is defined as the wavelength where solvent also absorbs UV.measurements must be avoided in this region.hence,it is difficult to determine the absorbance comes from analyte or solvent.for example methanol has UV cut off value of 205 nm.if our analyte is dissolved in methanol ,we should not fix our wavelength near to 205 nm.

Is This Answer Correct ?

11 Yes

0 No

Question { 2557 }

What is impurities in pharma analysis?

Answer

Impurities are unwanted chemicals that remain with the API or developed during formulation.
Thanks,
B.Gangatharan,
Caplin point laboratory,
Puducherry.

Is This Answer Correct ?

2 Yes

0 No

Question { Apotex, 2872 }

What are the different zones in stability according to ICH?

Answer

Zone I - Temperate zone
Zone II- MediterraneanSubtropical
Zone III-Hot dry zone
Zone IVa-Hot humid ropical
Zone IVb-Hothigher humidity
Thanks,
B.Gangatharan.

Is This Answer Correct ?

4 Yes

0 No

Question { Cipla, 3285 }

According to ICH,India is coming under which climatic zones?

Answer

Zone III & Zone IV b
Thanks,
B.Gangatharan

Is This Answer Correct ?

7 Yes

0 No

Question { 2536 }

How does GC did work?

Answer

Gc FID uses hydrogenairflame
In to which sample is passed to oxidise or burn organic molecules or hydrocarbons.it produces electrically charged particles or ions.(any hydrocarbon in sample will produce ions when they are burnt).the ions are collected and produce an electrical signal
Which is then measured.
Thanks,
B.Gangatharan,
Caplin point.

Is This Answer Correct ?

4 Yes

0 No

Question { Glenmark, 9869 }

Which detector is used in uv-vis spectrophotometer?

Answer

Photomultiplier tube is commonly used detector in uv spectrophotometer.
Thanks,
B.Gangatharan.

Is This Answer Correct ?

6 Yes

1 No

Question { Lupin, 2552 }

Why chromic acid wash is not recommended by USP for glassware cleaning?

Answer

According to USP chapter<1051>chromic acid is not recommended for glassware cleaning due to hazardous &toxic nature of the material.
Thanks,
Gangatharan.b
Caplin point,
Puducherry.

Is This Answer Correct ?

2 Yes

0 No

b.gangatharan