what is the primary purpose of fermentation in the absence
of oxygen?
which is known as biologic grignard reagent?
Garnet is the ore of which element?
ano po ba ang bermuda triangle? ano po ang nandito? meron po bang mga naitala na aksidente sa gawing ito? ano po ang scientific question nito?
Ethylene on ozonolysis gives which chemical compound?
Which enzymes in cows allow them to digest cellulose?
Explain how the production of rna called?
How does the formation of the enzyme-substrate complex?
Since pepsin is a gastric enzyme does it, has acid or basic optimum ph? What happen to pepsin when it passes to the duodenum?
The expression q=delta (e)-w is? What does it states?
What causes aromatic rings to stick to each other?
Is there difference between the initial and the final energy levels in catalyzed and non-catalyzed reactions?
I have given the protocol for the cyclodextrin glygosyl transferase assay: One ml of appropriately diluted enzyme sample was incubated at 60 °C for 15 min with 5 ml of 1% (w/v) gelatinized soluble starch in 50 mM, 7.0-pH Tris–HCl buffer. Reaction was terminated by boiling the reaction mixture for 3 min and reaction volume was made to 10 ml with distilled water. Two ml of above reaction mixture was withdrawn and mixed with 3 ml of Tris–HCl buffer, 5 ml of 125 mM Na2CO3, and 0.5 ml of phenolphthalein (25 mg phenolphthalein/100 ml absolute alcohol). Absorbance was measured at 550 nm. The percent decrease of sample was calculated with respect to control containing 5 ml of buffer, 5 ml of sodium carbonate and 0.5 ml of phenolphthalein. where Acontrol = absorbance of control and Atest = absorbance of sample. The amount of β-cyclodextrin (β-CD) produced was estimated from the standard graph of 0–500 μg/ml β-CD concentration against % decrease in absorbance. One unit of CGTase was defined as the amount of enzyme required to produce 1 μm of β-CD/min. Please can you suggest me the formula for the defination given in the last line