What is a transcription factor?

1 Answers  

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I have given the protocol for the cyclodextrin glygosyl transferase assay: One ml of appropriately diluted enzyme sample was incubated at 60 °C for 15 min with 5 ml of 1% (w/v) gelatinized soluble starch in 50 mM, 7.0-pH Tris–HCl buffer. Reaction was terminated by boiling the reaction mixture for 3 min and reaction volume was made to 10 ml with distilled water. Two ml of above reaction mixture was withdrawn and mixed with 3 ml of Tris–HCl buffer, 5 ml of 125 mM Na2CO3, and 0.5 ml of phenolphthalein (25 mg phenolphthalein/100 ml absolute alcohol). Absorbance was measured at 550 nm. The percent decrease of sample was calculated with respect to control containing 5 ml of buffer, 5 ml of sodium carbonate and 0.5 ml of phenolphthalein. where Acontrol = absorbance of control and Atest = absorbance of sample. The amount of β-cyclodextrin (β-CD) produced was estimated from the standard graph of 0–500 μg/ml β-CD concentration against % decrease in absorbance. One unit of CGTase was defined as the amount of enzyme required to produce 1 μm of β-CD/min. Please can you suggest me the formula for the defination given in the last line

0 Answers  

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what is niemann-pick disease and what are the disease symptoms?

2 Answers   Biocon,

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explain the working of monochromator unit?

3 Answers   Reddy Labs,

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What determines whether a B-cell will respond to an antigen?

1 Answers  

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What features do the helix and the sheet have in common?

4 Answers  

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What are antibodies?

2 Answers  

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How many sigma and pi bonds are present in nitrogen formula?

0 Answers  

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what are the different types of monoterpenes?

1 Answers   Ranbaxy,

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why ph is not more than 14?

0 Answers  

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Name the terminal electron acceptor during mitochondrial respiration?

1 Answers  

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what is Alzheimer's disease?what are its symptoms?

1 Answers  

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