why we use 96 well plate in ELISA plate reader?
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Explain the basic structure of collagen?
How can energy change in the formation of NACL be determined?
I have given the protocol for the cyclodextrin glygosyl transferase assay: One ml of appropriately diluted enzyme sample was incubated at 60 °C for 15 min with 5 ml of 1% (w/v) gelatinized soluble starch in 50 mM, 7.0-pH Tris–HCl buffer. Reaction was terminated by boiling the reaction mixture for 3 min and reaction volume was made to 10 ml with distilled water. Two ml of above reaction mixture was withdrawn and mixed with 3 ml of Tris–HCl buffer, 5 ml of 125 mM Na2CO3, and 0.5 ml of phenolphthalein (25 mg phenolphthalein/100 ml absolute alcohol). Absorbance was measured at 550 nm. The percent decrease of sample was calculated with respect to control containing 5 ml of buffer, 5 ml of sodium carbonate and 0.5 ml of phenolphthalein. where Acontrol = absorbance of control and Atest = absorbance of sample. The amount of β-cyclodextrin (β-CD) produced was estimated from the standard graph of 0–500 μg/ml β-CD concentration against % decrease in absorbance. One unit of CGTase was defined as the amount of enzyme required to produce 1 μm of β-CD/min. Please can you suggest me the formula for the defination given in the last line
what is the emperical formula of vitamin b12?
How does the UV absorption of specific amino acids aid biochemists?
What causes aromatic rings to stick to each other?
how the keratosulphate differs from other mucopolysaccharides?
What is the name of the dna duplication process?
Which type of chemical bond maintains the pairing of each chain in the dna molecule?
Why ethylene undergoes electrophilic addition reactions?
how waxes can be prepared?
what is schweizner's reagent?