BIOCHEMICAL ENGINEERING INSTRUMENTATION - EXAMPLE 29.4 : The resolution of separation, Rs for chromatography is given by the formula Rs = (difference in retention time) / (average width at the base). In a chromatogram, 3 peaks a, b and c are found. Average widths W at the bases of the solutes are : Wa = 20 s, Wb = 40 s, Wc = 30 s. Resolutions of separation, Rs for solutes b and c in comparison to a are 2 and 4 respectively. The differences in retention times T for b and c in comparison to a are (Tb - Ta) and (Tc - Ta), Ta = Tc - Tb : (a) Form 2 equations involving Rs as a function of Wa, Wb, Wc, Ta, Tb and Tc. (b) Find the values of Ta, Tb and Tc.



BIOCHEMICAL ENGINEERING INSTRUMENTATION - EXAMPLE 29.4 : The resolution of separation, Rs for chroma..

Answer / kangchuentat

BIOCHEMICAL ENGINEERING INSTRUMENTATION - ANSWER 29.4 : Reference formula Rs = (difference in retention time) / (average width at the base) is used. (a) First equation : Rs = (Tb - Ta) / [ (Wa + Wb) / 2 ] = 2 (Tb - Ta) / (Wa + Wb). Second equation : Rs = (Tc - Ta) / [ (Wa + Wc) / 2 ] = 2 (Tc - Ta) / (Wa + Wc). (b) Substitute Ta = Tc - Tb, Wa = 20 s, Wb = 40 s and Wc = 30 s into first equation and second equation. First equation : Rs = 2 = 2 [ Tb - (Tc - Tb) ] / (20 + 40) = (2 Tb - Tc) / 30, 2 Tb - Tc = 60. Second equation : Rs = 4 = 2 [ Tc - (Tc - Tb) ] / (20 + 30) = Tb / 25, Tb = 100 s. Substitute Tb = 100 s into first equation gives 2 Tb - Tc = 2 x 100 - Tc = 200 - Tc = 60, then Tc = 200 - 60 = 140 s. Then Ta = Tc - Tb = 140 - 100 = 40 s. The answer is given by Kang Chuen Tat; PO Box 6263, Dandenong, Victoria VIC 3175, Australia; SMS +61405421706; chuentat@hotmail.com; http://kangchuentat.wordpress.com.

Is This Answer Correct ?    0 Yes 0 No

Post New Answer

More Chemical Engineering Interview Questions

ACCOUNTING AND FINANCIAL ENGINEERING - EXAMPLE 34.30 : A biochemical engineering sales contract adopts Cost Recovery Method in its company accounting. Gross profit is realised only when cash collections exceed the total cost of goods sold. Let Y = Original Cost Recovered, Z = Gross Profit Realised. Its property was sold at A = $500k with cost of B = $300k. Its engineering accountant receives cash of C = $240k in first year, D = $180k in second year and E in third year. (a) Find the values of Y and Z in : (i) first year; (ii) second year; (iii) third year. (b) What is the exact value of E if all cash has been fully received from sales?

1 Answers  


pls send me model paper of NPCIL for executive trainee. and syllabus of executive trainee 2015.

0 Answers  


hi all i have doe cheical engg, but i have to go for iob po interview so can anybody suggest me abt the kind of qns that can be asked.

0 Answers   Indian Overseas Bank,


Explain what types of metals are typically removed via chemical precipitation?

0 Answers  


what is flooding in a column.How do you know flooding has occured?

2 Answers   Cairn,






What are the strategies acquired to cure tube vibration and exchangers in a shell?

0 Answers  


Question 107 - In N + 1 Rule in Quantum Chemistry, whenever a spin 1 / 2 nucleus is adjacent to N other nuclei, it is split into N + 1 distinct peaks. In 1 peak or singlet, there is only 1 magnitude. In 2 peaks or doublet, the ratio of magnitude of each peak is 1 : 1. In 3 peaks or triplet, the ratio of magnitude of each peak is 1 : 2 : 1. In 4 peaks or quartet, the ratio of magnitude of each peak is 1 : 3 : 3 : 1. In 5 peaks or quintet, the ratio of magnitude of each peak is 1 : 4 : 6 : 4 : 1. (a) By using binomial coefficients or Triangle of Pascal find the ratio of magnitude of each peak if 6 peaks exists. (b) How many adjacent nuclei are available in a spin 1 / 2 nucleus in such situation of 6 peaks?

1 Answers  


What does the catalytic converter on an automobile really do?

0 Answers  


Question 64 – According to a heuristic of chemical engineering plant design, assume a pressure difference dP = 4 psi for each 10-ft rise in elevation. A pump is needed to pump liquid from a storage tank at a lower elevation to a heating tank at a higher elevation with the elevation difference of 60 ft. (a) Find the pressure loss due to such elevation. (b) If the required minimum inlet pressure to the heating tank is 9 psi, with 1 control valve is installed between pump and heating tank, what is the dP minimum required for the control valve and the entrance to the heating tank when the heuristic mentions that at least 10 psi is required for the control valve? (c) The pressure at the inlet of the pump is 8 psi and the flowrate of the liquid produces pressure head of 50 psi. What is the total pressure produced by the pump? (d) Assume a pipeline dP of 2 psi / 100 ft for liquid flow in a pipe according to heuristic, what is the approximate maximum length of the pipe in ft that can be installed between the pump and the heating tank?

1 Answers  


Can condensate control in a reboiler cause water hammer problems?

0 Answers  


MICROBIOLOGICAL ENGINEERING - QUESTION 28.3 : In the calculation of the growth of bacteria, absorbance, A in spectrophotometry is used. According to Beer-Lambert Law, A = e x l x c where A is the absorbance of the solution (no unit), l is the distance of light travels through the solution (in cm), e is the molar absorptivity or the molar extinction coefficient [ in L / (mol.cm) ]. For a particular solute and fixed path length : As / Ao = Cs / Co where Ao is the observed signal for a known concentration Co, and As is the observed signal for a sample concentration Cs. (a) For a cell concentration of 560 cells / mL, a spectrophotometre gives an absorbance reading of 1.0. A mixture of concentration 3600000 cells / mL can be diluted in several operations, with each operation having a dilution of 1:20. How many dilutions should be made so that the concentration of this mixture can be calculated within a range of A = 0.0 to 1.0. (b) In another experiment, a sample tube of 1 cm in width is used. Let A = 0.06 and e = 0.0012 ml / (cell.cm). Find the cell concentration of the sample.

1 Answers  


ACCOUNTING AND FINANCIAL ENGINEERING - EXAMPLE 34.35 : A voluntary accountant is performing Du Pont Analysis for a charitable engineering organization. References for 3-step Du Pont formula are : (ROE) = (Net Income $) / (Equity $), where ROE is Return On Equity. (ROS) = (Net Income $) / (Sales $), where ROS is Return On Sales. (Asset Turnover) = (Sales $) / (Assets $). (Leverage) = (Assets $) / (Equity $). From the balance sheet and income statement, the company has Assets of $3753.19 this year and $3216.86 last year. Sales for this year is $2292.34. Let ROE = 0.37 and ROS = 0.21. (a) Calculate the Asset Turnover of the organization. (b) Find the value of the organizational Leverage. (c) Calculate the Net Income $ of the organization.

1 Answers  


Categories
  • Civil Engineering Interview Questions Civil Engineering (5085)
  • Mechanical Engineering Interview Questions Mechanical Engineering (4451)
  • Electrical Engineering Interview Questions Electrical Engineering (16632)
  • Electronics Communications Interview Questions Electronics Communications (3918)
  • Chemical Engineering Interview Questions Chemical Engineering (1095)
  • Aeronautical Engineering Interview Questions Aeronautical Engineering (239)
  • Bio Engineering Interview Questions Bio Engineering (96)
  • Metallurgy Interview Questions Metallurgy (361)
  • Industrial Engineering Interview Questions Industrial Engineering (259)
  • Instrumentation Interview Questions Instrumentation (3014)
  • Automobile Engineering Interview Questions Automobile Engineering (332)
  • Mechatronics Engineering Interview Questions Mechatronics Engineering (97)
  • Marine Engineering Interview Questions Marine Engineering (124)
  • Power Plant Engineering Interview Questions Power Plant Engineering (172)
  • Textile Engineering Interview Questions Textile Engineering (575)
  • Production Engineering Interview Questions Production Engineering (25)
  • Satellite Systems Engineering Interview Questions Satellite Systems Engineering (106)
  • Engineering AllOther Interview Questions Engineering AllOther (1379)