ex:this product used c18 column but same product used c8 column what happend ?

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ex:this product used c18 column but same product used c8 column what happend ?..

Answer / ravendranadhareddy

i think rt changed

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ex:this product used c18 column but same product used c8 column what happend ?..

Answer / rashmikant bhavsar

retention time shoud be changed .if you c8 column rt is 4 min ,and used c18 column hplc condition same as used c8 colum so change r.t. comparision c8 column ,rt shoud be 6 min approxy

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