Question { Aurobindo, 201530 }

Why we use caffeine for HPLC calibration in Detector wave
ength accuracy test

Answer

i said one my interview it is low cost,multiple wave lengths but these are not correct he said.any person know exact reason tell me.


but i think in uv spectroscopy some electronic bonding,non bonding transitions by c-n,c=c,c=n,n-o interactions.then it gives different wavelenghths with in the uv region only other compounds its not easily detectable