Question { Reddy Labs, 187757 }

Why we use caffeine for HPLC calibration in Detector wave
ength accuracy test

Answer

caffeiene having different wavelents it having 2 maximas
and 1 minima.
1st maxima is 205nm
2nd maxima is 273nm
minima is 245nm
and it is primary reference standard and also suggested in
pharmacopiea.
It is very stable, we can use a long time foranalysis
i.e. it is not degradable