Question { Reddy Labs, 45148 }

How to fix the concentration for RS by HPLC

Answer

Formula=Estimation LOQ concentration (PPM)X1/Desired LOQ concentration (%)X100.
For example as per ICHQ3B our product Reporting threshold was 0.05% this is called Desired LOQ concentration.First initially we fix sample concentration as 500 ppm.This is 100%,as per this, 0.05% LOQ ppm was 0.25 ppm this is Estimated LOQ concentration.

Question { 16671 }

how to prepare 25 ppm acetone. explian with formula

Answer

first we prepare 1000 ppm(mgml).
1000 ppm acetone standard solution preparation:-10 mg of acetone solvent is taken in 10 ml vf and make up with suitable diluent.
25 ppm preparation:- 0.25 ml of 1000 ppm solution is taken in a 10 ml vf and make up with a that suitable diluent.
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