Which vitamins participate, in coenzyme form, in reactions of the tricarboxylic acid cycle?

2119

A blastomere was removed from an 8-cell human embryo, and its beta-globin gene was amplified by PCR. The beta-globin DNA was treated with an enzyme that cuts on either side of and within the normal allele (producing a small fragment), but not within the sickle cell allele (producing a large fragment). Electrophoresis of the treated DNA from the blastomere demonstrated the presence of both a large and small fragment derived from the beta-globin genes. How should you interpret this test?

2031

Can angiotensin-converting enzyme (ACE) inhibitors and angiotensin receptor blockers cause urticarial skin eruptions?

2496

Compare and contrast feedback inhibition and enzyme repression?

9747

What are the enzymes produced by streptococci?

2062

The elution volume of an enzyme on a gel filtration column can be predicted from a) its enzyme activity b) its protein absorbance at 280 nm c) subunit composition and monomer molecular mass(es) d) Choices a) and b) are both correct.

3211

What is enzyme-ligand interaction?

1943

I have given the protocol for the cyclodextrin glygosyl transferase assay: One ml of appropriately diluted enzyme sample was incubated at 60 °C for 15 min with 5 ml of 1% (w/v) gelatinized soluble starch in 50 mM, 7.0-pH Tris–HCl buffer. Reaction was terminated by boiling the reaction mixture for 3 min and reaction volume was made to 10 ml with distilled water. Two ml of above reaction mixture was withdrawn and mixed with 3 ml of Tris–HCl buffer, 5 ml of 125 mM Na2CO3, and 0.5 ml of phenolphthalein (25 mg phenolphthalein/100 ml absolute alcohol). Absorbance was measured at 550 nm. The percent decrease of sample was calculated with respect to control containing 5 ml of buffer, 5 ml of sodium carbonate and 0.5 ml of phenolphthalein. where Acontrol = absorbance of control and Atest = absorbance of sample. The amount of β-cyclodextrin (β-CD) produced was estimated from the standard graph of 0–500 μg/ml β-CD concentration against % decrease in absorbance. One unit of CGTase was defined as the amount of enzyme required to produce 1 μm of β-CD/min. Please can you suggest me the formula for the defination given in the last line

2153

how to analyse serum cardiac marker, cardiac enzymes, troonin and myoglobin?

2253

What are some biological examples in which lysosomic enzymes play fundamental role?

970

What are the enzymes used for labeling of antibodies?

380

Name two enzymes that have been employed for elisa?

504

What happen to a denaturated enzyme regarding its functionality? How that result can be explained with the help of the lock and key model?

1054

What are allosteric enzymes?

1032

What is the importance of enzymes for the living beings?

1119