State two advantages of using immobilised enzymes.
Answers were Sorted based on User's Feedback
Answer / kush
1) Better availability of active sites to the substrate
molecules, due to the fixed position of the enzymes.
2) Wear and tear of the enzymes due to shear forces acting
in the dissolved state on the enzyme.
| Is This Answer Correct ? | 55 Yes | 17 No |
Answer / suganthi
Easy separation from reaction mixture, providing the
ability to control reaction times and minimize the enzymes
lost in the product.
Re-use of enzymes for many reaction cycles, lowering the
total production cost of enzyme mediated reactions.
Ability of enzymes to replace multiple standard chemical
steps and provide enatomerically pure products.
| Is This Answer Correct ? | 35 Yes | 3 No |
Answer / rishu
Immobilized enzymes are enzymes which are either covalently
bound or absorbed onto the surface of an insoluble support.
Immobilized enzymes have several advantages over the
soluble enzyme.
Convenience: Miniscule amounts of protein dissolve in the
reaction, so workup can be much easier. Upon completion,
reaction mixtures typically contain only solvent and
reaction products.
Economical: The immobilized enzyme is easily removed from
the reaction making it easy to recycle the biocatalyst.
Stability: Immobilized enzymes typically have greater
thermal and operational stability than the soluble form of
the enzyme.
| Is This Answer Correct ? | 36 Yes | 7 No |
Answer / souneek chakraborty
1.Using the immobilised enzyme is cost effective as it
facillates Down stream processing.
2.The can be reused several times.
| Is This Answer Correct ? | 12 Yes | 3 No |
Answer / naveen kumar m
I accept the above points. every one mentioning about
higher productivity but they could not give reasons, its
due to higher turn over number of immobilized enzyme.
2. we can reduce the wastage of enzyme
| Is This Answer Correct ? | 8 Yes | 5 No |
Answer / kamana emmanuel
1.because of its binding with a matrix, the immobilized
enzyme has better stability in many cases
2.Efficieny if immobilized enzyme is better and therefore
high product yield
| Is This Answer Correct ? | 10 Yes | 8 No |
Answer / kathir
1)to keep the enzyme in inactivate form. 2)use in future.
| Is This Answer Correct ? | 10 Yes | 25 No |
what happen when there is absence of sulfur cantaining amino acid in protein synthesis?
How DNA is being Transplanted?
How to calculate protein percentage in lowry method?
What is the basic difference between quantitative and quantal assays?
1 Answers Biological.e.limited,
why we use 96 well plate in ELISA plate reader?
what is biophysics?
How the dose for children is being estimated based on preclinical data?
Sir i completed m.sc.biotech but for good career i want to do some diploma courses for good job . Will you please suggest me the coures
if u've any queries or need more info about biocon interviews. u jus mail to raaju_kaarthikeyan@yahoo.co.in
I am a final year student of bio_technology.I dont know how to make my profile?where is the opportunities for my studies?how is the interview flow?
Name the dye commonly used as the counterstain in the Gram staining process.
What is Proteinase K?