Name an a marker gene other than kanamycin resistance gene
used in plant tissue culture and why it is used now a days?
- 1 Answers
- 3525 Views
- I also Faced
- E-Mail Answers
Answer / ambalika
A new selectable marker system was adapted for use in Agrobacterium-mediated transformation of rice (Oryza sativa L.). This selection system utilizes the pmi gene encoding for phosphomannose-isomerase that converts mannose-6-phosphate to fructose-6-phosphate. Only transformed cells were capable of utilizing mannose as a carbon source. Transgenic rice plants regenerated from selected transformed immature embryo-derived calli on media containing various concentrations of mannose. The highest transformation frequency of 6.0% was obtained when a combination of 25 g/l mannose and 5 g/l sucrose was used. Molecular and genetic analyses showed that the plants contained the pmi gene and the gene was transmitted to the progeny in Mendelian fashion. The results indicated that the mannose selection system, which was devoid of the disadvantages of antibiotic or herbicide selection, could be used for rice Agrobacterium-mediated immature embryo transformation.
| Is This Answer Correct ? | 0 Yes | 0 No |
what are the conditions and time requirements for carrying out the various steps involved in northern blotting?
why do we use DBM instead of NCF in the northern blotting and what are the pre-treatments required for this paper to be used?
Is it possible to express the genes of prokariots into eukariots?
i have done Msc Biotechnology from Chennai university in India.very shortly iam going to relocate to USA getting the dependant visa as my husband is going to usa. can i do job in USA
Hi i finished M.tech biotechnology in trichy.can i get biotechnology lecturer jobs at arts and science colleges temporarily please ans me
Name the dye commonly used as the counterstain in the Gram staining process.
The absorbance of protein at 280 nm is due to which amino acid?
What are the different types of bio technology? Explain.
how is beta propiolactone gets inactivated,please tell me the factors which affects its assay
2 Answers Biocon, Indian Immunologicals,
Difference between Totipotent and pluripotent stem cells?
i have pcb and i am all set to do biotech so i wanted to know is this a successful option which has its perks??
is biotechnology going to be ever green branch
Micro Biology 
