Interested to Buy Any Domain ? << Click Here >> for more details...
Name an a marker gene other than kanamycin resistance gene
used in plant tissue culture and why it is used now a days?
- 1 Answers
- 4225 Views
- I also Faced
- E-Mail Answers
Answer Posted / ambalika
A new selectable marker system was adapted for use in Agrobacterium-mediated transformation of rice (Oryza sativa L.). This selection system utilizes the pmi gene encoding for phosphomannose-isomerase that converts mannose-6-phosphate to fructose-6-phosphate. Only transformed cells were capable of utilizing mannose as a carbon source. Transgenic rice plants regenerated from selected transformed immature embryo-derived calli on media containing various concentrations of mannose. The highest transformation frequency of 6.0% was obtained when a combination of 25 g/l mannose and 5 g/l sucrose was used. Molecular and genetic analyses showed that the plants contained the pmi gene and the gene was transmitted to the progeny in Mendelian fashion. The results indicated that the mannose selection system, which was devoid of the disadvantages of antibiotic or herbicide selection, could be used for rice Agrobacterium-mediated immature embryo transformation.
| Is This Answer Correct ? | 0 Yes | 0 No |
Post New Answer View All Answers
Give an example for electrophilic substitution reaction?
Hi guys.My friend has visa appointment in this month but the problem is she applied for the university with her old toefl score and she wrote toefl after she applied and her toefl date expired last month but she did'nt received her official score report till now.Is there any problem if she attended the visa interview with his old score
What are the possible dangers that bio technology and bio medicine faces on the advanced scientific methods of food products?
what type of questions are asked in interview for biotechnology student?
what are the shine-dalgarno sequences in organism other than E.coli.?
What do you mean by bio-media?
Hi i finished M.tech biotechnology in trichy.can i get biotechnology lecturer jobs at arts and science colleges temporarily please ans me
Dubai is desert, how it is possible it is becoming greenery?
What are the problems we could face by using this genetic engineering tools?
Sir i am doing my btech biotech at dy patil pune nd due to some prob i need to shift backbto surat so is it possible to transfer at bsc biotech college in surat
Why do you use dbm instead of ncf in the northern blotting and what are the pre-treatments required for this paper to be used?
What does GMO stands for and what importance does it has in bio-technology field?
At is the difference between transfection and transduction?
i got 59/120 in toefl. i have also 90% in 12th science.am i eligible for going usa for biotechnology? i ll also take SAT exam. i want to apply pensylvania,new jursey or newyork . plz hep me.
could u please say me the job opportunities for M.Sc biotech students in India
