The absorbance of protein at 280 nm is due to which amino
acid?
Answers were Sorted based on User's Feedback
Its due to amino acids containing an aromatic ring in their
structure...Eg. Tryptophan, tyrosine and phenylalanine
Is This Answer Correct ? | 63 Yes | 4 No |
Answer / moumita ghosal
it is due to tryptophan and tyrosine.phenylalanine absorbs
at 260nm. but the former two has absorbance maxima at
280nm. absorbance is due to resonance phenomena of the
rings present in these amino acids.
Is This Answer Correct ? | 23 Yes | 1 No |
Answer / jason
Cystine (two cysteines joined in a disulfide bridge) also
absorb at 280nm along with the aforementioned tryptophan
and tyrosine.
Is This Answer Correct ? | 8 Yes | 15 No |
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