Question 89 - A hemocytometer is a device that is used for counting cells. In an engineering experiment, 100 microlitres of cell suspension is diluted with 50 microlitres of Trypan blue dye. Only death cells appear blue in color when stained with the dye. There are 57 cells detected in a hemocytometer, where 5.3 % of them appear blue when the chamber of the meter is placed under a microscope. Each square of a chamber can contain 0.0001 mL of liquid. (a) Calculate the number of viable cells. (b) The cells ocupied 5 squares. Calculate the average number of viable cells / square. (c) Calculate the dilution factor of the cell suspension by using the formula : Dilution = final volume / initial volume. (d) Calculate the concentration of viable cells / mL by using the formula : Concentration = (Average number of viable cells / square) x dilution x (square / volume).

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What is the import procurement cycle? What are the customization steps in sap?

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What is a good approximation for the heat transfer coefficient of hydrocarbons inside 3/4" tubes?

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BIOPROCESS ENGINEERING - EXAMPLE 14.2 : An aqueous solution with 2.5 g of a protein dissolved in 600 cubic centimeters of a solution at 20 degree Celsius was placed in a container that has a water-permeable membrane. Water permeated through the membrane until the h - level of the solution was 0.9 cm above the pure water. (a) Calculate the absolute temperature of the solution, T in Kelvin, where T (Kelvin) = T (degree Celsius) + 273.15. (b) Calculate the osmotic pressure, P of the solution by using the formula P = hrg where h is level of the solution, r is density of water with 1000 kg per cubic meter, g = 9.81 N / kg as gravitational acceleration. (c) Calculate the concentration of the protein solution, C in kg / cubic meter. (d) Calculate the molecular weight of the protein, (MW) = CRT / P where R = 8.314 Pa cubic meter / (mol K) as ideal gas constant.

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what is the hearth and arch pressure in furnace?

0 Answers   IFFCO,

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Explain how can one determine if a particular solid can be fluidized as in a fluidized bed?

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Define viscosity?

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ENGINEERING PHYSICS - EXAMPLE 30.5 : (a) Let | A > = (Aa Ab Ac), | B > = (Ba Bb Bc), | C > = (Ca Cb Cc). Find | A > + | C > - | B > in term of Aa, Ab, Ac, Ba, Bb, Bc, Ca, Cb and Cc. (b) Let d | E > = d (Ea Eb Ec) = (d Ea d Eb d Ec). If | E > = (6 7 8), find the value of 10 | E >.

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A 1.5 weight % aqueous salt solution is concentrated to 4 weight % in a single-effect evaporator. The feed rate to the evaporator is F = 7500 kg / h and the feed is at 85 degree Celsius. The evaporator operates at 1 bar. By assuming that only pure solvent of water exists in the form of vapor from the feed, calculate the flow rate of such vapor V.

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How can the particle size distribution be determined in a given bulk solid?

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ACCOUNTING AND FINANCIAL ENGINEERING - EXAMPLE 34.32 : In the calculation of the total cost of production of an engineering material, a linear equation Y = MX + C could be applied. Let T = total cost, F = fixed cost, V = variable cost, Q = quantity produced, W = variable cost per unit produced. Form a linear equation that relates : (a) T, F and V; (b) V, Q and W.

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What is the difference between multi grade filter and dual media filter?

1 Answers   Dalkia, Oil and Gas Jobs,

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