BIOCHEMICAL ENGINEERING INSTRUMENTATION - EXAMPLE 29.3 : In thin layer chromatography (TLC), let retention factor Rf = (distance traveled by solute) / (distance traveled by solvent). Silica gel is used as stationary phase which is more polar than the hexane solvent as mobile phase. If the Rf values of 3 solutes A, B and C are 1.5, 0.75 and 1 respectively, compare the polarities of A, B and C with reasons.
BIOCHEMICAL ENGINEERING INSTRUMENTATION - ANSWER 29.3 : Polarities : A < C < B. A is least polar since it travels farthest with the mobile phase, whereas B is most polar as it is attracted to the stationary phase and travels least. The answer is given by Kang Chuen Tat; PO Box 6263, Dandenong, Victoria VIC 3175, Australia; SMS +61405421706; chuentat@hotmail.com; http://kangchuentat.wordpress.com.
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ACCOUNTING AND FINANCIAL ENGINEERING - EXAMPLE 34.13 : (i) In the Present Value Multiplication Rule, let PV = present value, Ra = interest rate for first discount, A = duration for first discount; Rc = interest rate for second discount, C = duration for second discount. Let PV = [ 1 / (1 + Ra) ^ A ] [ 1 / (1 + Rc) ^ C ] where ^ is the symbol of power : 3 ^ 2 = 3 x 3, 2 ^ 3 = 2 x 2 x 2. (a) For discounts involving 8 % / year for 3 years and 10 % / year for 9 years, find the value of PV. (b) If Re = interest rate for third discount, E = duration of third discount, form a mathematical equation of PV as a function of A, C, E, Ra, Rc, Re. Note : Discounts are available in the purchase of certain biochemical engineering instruments. (ii) Let R = nominal interest rate related to growth rate of money, r = real interest rate related to growth rate of purchase power. If I = inflation, where the unit of R, r and I is %, find the mathematical relatonship of r as a function of R and I.
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Water flows through a pipe with circular cross sectional area at the rate of V / t = 80 L / s where V is the volume and t is time. Let Av = 80 L / s where A is cross sectional area and v is velocity of fluid. For point 1, the radius of the pipe is 16 cm. For point 2, the radius of the pipe is 8 cm. Find (a) the velocity at point 1; (b) the velocity at point 2; (c) the pressure at point 2 by using Bernoulli’s equation where P Rgy 0.5 RV = constant. P is the pressure, R = density of fluid, V = square of fluid’s velocity, g = gravitational constant of 9.81 N / kg and y = 2 m = difference of height at 2 points. The pressure of point 1 is 180 kPa.
Question 88 - In the calculation of the growth of bacteria, colony forming unit (CFU) in serial dilution is used. In a laboratory, viable count assay is used to estimate CFU. Formula applied is CFU / mL = (number of colonies x dilution) / (amount plated, in unit mL). Acceptable plate count is either between 20 and 200 or between 30 and 300 according to 2 different references. A wastewater sample of 200 ml is added to and mixed with 1.8 L of sterile water. Another 200 ml of the mixture is added to and mixed with 1.8 L of sterile water. (a) Calculate the dilution of first mixture and the dilution of the second mixture. (b) 100 microlitres of wastewater samples from the first mixture and the second mixture are placed separately on 2 different alga plates. The first plate has 250 colonies and the second plate has 23 colonies. Calculate the average CFU / mL.
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