MICROBIOLOGICAL ENGINEERING - QUESTION 28.2 : A hemocytometer is a device that is used for counting cells. In an engineering experiment, 100 microlitres of cell suspension is diluted with 50 microlitres of Trypan blue dye. Only death cells appear blue in color when stained with the dye. There are 57 cells detected in a hemocytometer, where 5.3 % of them appear blue when the chamber of the meter is placed under a microscope. Each square of a chamber can contain 0.0001 mL of liquid. (a) Calculate the number of viable cells. (b) The cells occupied 5 squares. Calculate the average number of viable cells / square. (c) Calculate the dilution factor of the cell suspension by using the formula : Dilution = final volume / initial volume. (d) Calculate the concentration of viable cells / mL by using the formula : Concentration = (Average number of viable cells / square) x dilution x (square / volume).



MICROBIOLOGICAL ENGINEERING - QUESTION 28.2 : A hemocytometer is a device that is used for counting ..

Answer / kangchuentat

MICROBIOLOGICAL ENGINEERING - ANSWER 28.2 : (a) Number of death cells = Total cells x % of death cells / 100 = 57 x 5.3 / 100 = 3.021 rounded to 3 cells. Number of viable cells = Total cells - number of death cells = 57 - 3 = 54 cells. (b) Average number of viable cells / square = 54 / 5 = 10.8 cells / square. (c) Dilution = final volume / initial volume = (100 + 50) microlitres / 100 microlitres = 1.5. (d) Concentration = (Average number of viable cells / square) x dilution x (square / volume) = 10.8 cells x 1.5 x (1 / 0.0001 mL) = 162000 cells / mL. The answer is given by Kang Chuen Tat; PO Box 6263, Dandenong, Victoria VIC 3175, Australia; SMS +61405421706; chuentat@hotmail.com; http://kangchuentat.wordpress.com.

Is This Answer Correct ?    0 Yes 0 No

Post New Answer

More Chemical Engineering Interview Questions

BIOCHEMICAL ENGINEERING INSTRUMENTATION - EXAMPLE 29.2 : (a) In order to determine the slurry volume (L) in chromatography, arrange the formula using the following parameters : slurry concentration (%), slurry volume (L), settled chromatography medium volume (L), 100. (b) In order to determine the chromatography medium volume (L) in gel filtration chromatography, arrange the formula using the following parameters : sample volume to be processed (L), chromatography medium volume (L), sample volume in percent of total column volume (%), 100.

1 Answers  


PLZ ANYONE SEND ME PLACEMENT PAPERS OF I.O.C.L MY ID---- gaurav.nitw.5609@gmailcom

0 Answers   IOCL,


Can any one send me the sample test papers of IOCL entry exam to be held on 6th july 2008. Also plz send the interview questions that cud probably be asked........i wud be highly obliged if u can send a few test paers and other stuff @ inderjeetd@gmail.com.........Inderpreet

0 Answers   IOCL,


ACCOUNTING AND FINANCIAL ENGINEERING - EXAMPLE 34.20 : Assume an engineer buys a $1 bond in period T while the nominal interest rate is R. The inflation rate at T + 1 is anticipated to be I. (a) If the bond is redeemed in period T + 1, how much money will the buyer engineer receive, in term of R, which is not affected by inflation? (b) Find the present value, PV of the proceeds from the bond, in term of R and I. (c) If the bond is redeemed in period T + 1, calculate the real growth or real value of the money that the buyer engineer will receive, in term of r = real interest rate, which is affected by inflation. (d) From the answers in (b) and (c), find the values of x, y and z in the following Fisher equation : (1 + x) = (1 + y) / (1 + z), in term of r, R and I.

1 Answers  


Define an additive?

0 Answers  






What are the assumptions made on conservation equation?

0 Answers  


What is composition of 18/8 stainless steel?

1 Answers  


I HAVE DOMAIN KNOWLEDGE IN PROCESS MANUFACTURINGAND I GOT AN APPARTUNITY AS A BUSINESS ANALYST AND I DNT HAVE ANY KNOWLEDGE ON ORACLE APPS/GIVE ME ANY VALUBLE SUGGESTION?

0 Answers   Satyam,


How much waterproofing compound in one Sqm Plastering (thick.12mm)

0 Answers   Ess Pee Construction,


Name the some common problems associated with bellow expansion joints?

0 Answers  


What is Vacuum? If pressure is less than that of atmospheric, can we call it vacuum? If so some places have less atmospheric pressures, can we call people living there are living in vacuum?

5 Answers   ISRO, TCS,


why PH scale range is 14? why it is not less or not greater than 14? solution of HCl or hydrogen containing liquid has a PH. but if the solution is KMnO4 here not hydrogen power does it PH? explain plz....

1 Answers  


Categories
  • Civil Engineering Interview Questions Civil Engineering (5085)
  • Mechanical Engineering Interview Questions Mechanical Engineering (4451)
  • Electrical Engineering Interview Questions Electrical Engineering (16632)
  • Electronics Communications Interview Questions Electronics Communications (3918)
  • Chemical Engineering Interview Questions Chemical Engineering (1095)
  • Aeronautical Engineering Interview Questions Aeronautical Engineering (239)
  • Bio Engineering Interview Questions Bio Engineering (96)
  • Metallurgy Interview Questions Metallurgy (361)
  • Industrial Engineering Interview Questions Industrial Engineering (259)
  • Instrumentation Interview Questions Instrumentation (3014)
  • Automobile Engineering Interview Questions Automobile Engineering (332)
  • Mechatronics Engineering Interview Questions Mechatronics Engineering (97)
  • Marine Engineering Interview Questions Marine Engineering (124)
  • Power Plant Engineering Interview Questions Power Plant Engineering (172)
  • Textile Engineering Interview Questions Textile Engineering (575)
  • Production Engineering Interview Questions Production Engineering (25)
  • Satellite Systems Engineering Interview Questions Satellite Systems Engineering (106)
  • Engineering AllOther Interview Questions Engineering AllOther (1379)