Answer / kangchuentat

GENETIC ENGINEERING - ANSWER 27.2 : (a) From I as origin, J - I = 8 %, K - J = 10 %, K - I = 18 %, then I ... 8 % ... J ... 10 % ... K. (b) There are 22 genes between b and y, separated by 3 % recombination between 2 adjacent genes. Percentage recombination between b and y = (22 + 1) x 3 % = 69 %. The answer is given by Kang Chuen Tat; PO Box 6263, Dandenong, Victoria VIC 3175, Australia; SMS +61405421706; chuentat@hotmail.com; http://kangchuentat.wordpress.com.

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MICROBIOLOGICAL ENGINEERING - QUESTION 28.2 : A hemocytometer is a device that is used for counting cells. In an engineering experiment, 100 microlitres of cell suspension is diluted with 50 microlitres of Trypan blue dye. Only death cells appear blue in color when stained with the dye. There are 57 cells detected in a hemocytometer, where 5.3 % of them appear blue when the chamber of the meter is placed under a microscope. Each square of a chamber can contain 0.0001 mL of liquid. (a) Calculate the number of viable cells. (b) The cells occupied 5 squares. Calculate the average number of viable cells / square. (c) Calculate the dilution factor of the cell suspension by using the formula : Dilution = final volume / initial volume. (d) Calculate the concentration of viable cells / mL by using the formula : Concentration = (Average number of viable cells / square) x dilution x (square / volume).

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GENETIC ENGINEERING - EXAMPLE 27.4 : According to Hardy-Weinberg Equation, p x p + 2 x p x q + q x q = 1 where p = dominant allele frequency and q = recessive allele frequency. Let p + q = 1. Fraction of population has 2 copies of the p gene = p x p. Fraction of population has 2 copies of the q gene = q x q. Fraction of population has a copy of p gene and a copy of q gene = 2 x p x q. In a small town, the allele frequency is q = 0.2 for a recessive gene, the delta-32 mutation, that gives human protection from HIV infection. (a) Find the allele frequency of a dominant gene, p. (b) What percent of the population has at least a copy of the gene that cause the population either immune to HIV or less susceptible to the disease?

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