Question 92 - (a) In order to determine the slurry volume (L) in chromatography, arrange the formula using the following parameters : slurry concentration (%), slurry volume (L), settled chromatography medium volume (L), 100. (b) In order to determine the chromatography medium volume (L) in gel filtration chromatography, arrange the formula using the following parameters : sample volume to be processed (L), chromatography medium volume (L), sample volume in percent of total column volume (%), 100.
Answer 92 - (a) By fraction of concentration, [ settled chromatography medium volume (L) ] / [ slurry volume (L) ] = slurry concentration (%) / 100. Then [ slurry volume (L) ] = [ settled chromatography medium volume (L) ] / [ slurry concentration (%) / 100 ]. (b) By fraction of volume, [ sample volume to be processed (L) ] / [ chromatography medium volume (L) ] = sample volume in percent total volume (%) / 100. Then chromatography medium volume (L) = [ sample volume to be processed (L) ] / [ sample volume in percent of total column volume (%) / 100 ]. The answer is given by Kang Chuen Tat; PO Box 6263, Dandenong, Victoria VIC 3175, Australia; SMS +61405421706; chuentat@hotmail.com; http://kangchuentat.wordpress.com.
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ACCOUNTING AND FINANCIAL ENGINEERING - EXAMPLE 34.13 : (i) In the Present Value Multiplication Rule, let PV = present value, Ra = interest rate for first discount, A = duration for first discount; Rc = interest rate for second discount, C = duration for second discount. Let PV = [ 1 / (1 + Ra) ^ A ] [ 1 / (1 + Rc) ^ C ] where ^ is the symbol of power : 3 ^ 2 = 3 x 3, 2 ^ 3 = 2 x 2 x 2. (a) For discounts involving 8 % / year for 3 years and 10 % / year for 9 years, find the value of PV. (b) If Re = interest rate for third discount, E = duration of third discount, form a mathematical equation of PV as a function of A, C, E, Ra, Rc, Re. Note : Discounts are available in the purchase of certain biochemical engineering instruments. (ii) Let R = nominal interest rate related to growth rate of money, r = real interest rate related to growth rate of purchase power. If I = inflation, where the unit of R, r and I is %, find the mathematical relatonship of r as a function of R and I.
GENETIC ENGINEERING - EXAMPLE 27.3 : (a) Male with genotype GGmm and phenotype gray wingless mates with female with genotype ggMM and phenotype black winged in fruit flies. G is dominant to g in color. M is dominant to m in wing shape. If the actual distribution of the second generation of the fruit flies was as follow : 890 gray wingless, 900 black winged, 115 gray winged, 95 black wingless, calculate the recombination frequency between the two genes and distance in recombination units. Let 1 map unit = 1 % recombination. (b) A DNA molecule has 180 base pairs and 20 % adenine. How many cytosine nucleotides are present in this molecule of DNA?
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GENETIC ENGINEERING - EXAMPLE 27.2 : (a) Three genes, I, J and K are available. All these genes are linked with respect to one another. If the percent recombination between I and J is 8 %, that between J and K is 10 %, and that between K and I is 18 %, what is the order of the gene? (b) Twenty six genes, a, b, c, d, e, f, ... x, y and z are available. All these genes are linked with respect to one another. If the percent recombination between a and b is 3 %, between b and c is 3 %, between c and d is 3 %, ... between w and x is 3 %, between x and y is 3 %, between y and z is 3 %, then what is the percentage recombination between b and y?
Dear guys we adding hypo at climax plant and that water is inlet to DM plant so with hypo water that can affect my resins of cation and anion. if it is affect life of my anion and cation so what kind of steps should be take.
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