Answer / divya sharma

The major limitation of blotting procedures is the length
of time needed and the fact that they can accommodate only
one probe at a time.

Answer / mehul

microarray technology could be used as a source of
alternative

Answer / abe

Blotting techniques show many limitations in comparison to other molecular techniques. It depends what you're attempting to look for, it's very situational.

- They take up alot of time
- They degrade protein tertiary structure
- They show limited quantitative abilities
- They show lower specificity and sensitivity compared to other techniques.

If you're looking for gene expression I would say reverse-transcriptase PCR is a more appropriate alternative.

Here is a good article discussing these limitations:

http://www.nature.com/jid/journal/v133/n7/pdf/jid2013216a.pdf?wt.ec_id=jid-201307

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