What types of things happen to proteins during posttranslational processing (hydroxylation, etc.)?
1 3302What are the structural and stability consequences of electron delocalization in amides (peptide bonds)?
1 3788Post New Bio Chemistry Questions
In double bond, how many sigma and Pi bonds are present in it?
Who were james watson, francis crik and maurice wilkins?
All technical,written test questions
How the production of rna called?
How does a random coil differ from an irregularly structured region?
What are respectively some remarkable functions of immunoglobulin?
I have given the protocol for the cyclodextrin glygosyl transferase assay: One ml of appropriately diluted enzyme sample was incubated at 60 °C for 15 min with 5 ml of 1% (w/v) gelatinized soluble starch in 50 mM, 7.0-pH Tris–HCl buffer. Reaction was terminated by boiling the reaction mixture for 3 min and reaction volume was made to 10 ml with distilled water. Two ml of above reaction mixture was withdrawn and mixed with 3 ml of Tris–HCl buffer, 5 ml of 125 mM Na2CO3, and 0.5 ml of phenolphthalein (25 mg phenolphthalein/100 ml absolute alcohol). Absorbance was measured at 550 nm. The percent decrease of sample was calculated with respect to control containing 5 ml of buffer, 5 ml of sodium carbonate and 0.5 ml of phenolphthalein. where Acontrol = absorbance of control and Atest = absorbance of sample. The amount of β-cyclodextrin (β-CD) produced was estimated from the standard graph of 0–500 μg/ml β-CD concentration against % decrease in absorbance. One unit of CGTase was defined as the amount of enzyme required to produce 1 μm of β-CD/min. Please can you suggest me the formula for the defination given in the last line
How does hplc differ from normal column chromatography, and what are its advantages?
What is chemical formula for borax?
What are respectively some remarkable functions of reverse transcriptase?
Covalent bond is directional therefore which covalent property is shown?
Explain what is the name of the dna duplication process? What is the main enzyme that participates in it?
What are the drawbacks of tissue slices technique?
What is the molecular formula, structure, and bond angle of phosphorous?
Which is the most Abundant Alkaline earth element?