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Why can it be said that the enzymatic action is highly specific?
What is the structure of phosphorous?
What is the molecular formula, structure, and bond angle of phosphorous?
What is meant by substrates of enzymatic reactions?
What is the element in third series the element with maximum oxidation state?
What are pentoses? To what organic group do pentoses belong?
Explain what does thermodynamics helps in predicting?
Explain which type of chemical bond maintains the pairing of each chain in the dna molecule?
define standard feat of formation?
mutarotation is observed when phenol and pyridine both are present but phenol or pyridine alone cannot show mutarotation-explain why?
What do you like LEAST & MOST about your current position?
Define polar covalent bond?
What is the structure of cscl and give the co-ordination number of Cscl?
Who proposed law of mass action? What does it states?
I have given the protocol for the cyclodextrin glygosyl transferase assay: One ml of appropriately diluted enzyme sample was incubated at 60 °C for 15 min with 5 ml of 1% (w/v) gelatinized soluble starch in 50 mM, 7.0-pH Tris–HCl buffer. Reaction was terminated by boiling the reaction mixture for 3 min and reaction volume was made to 10 ml with distilled water. Two ml of above reaction mixture was withdrawn and mixed with 3 ml of Tris–HCl buffer, 5 ml of 125 mM Na2CO3, and 0.5 ml of phenolphthalein (25 mg phenolphthalein/100 ml absolute alcohol). Absorbance was measured at 550 nm. The percent decrease of sample was calculated with respect to control containing 5 ml of buffer, 5 ml of sodium carbonate and 0.5 ml of phenolphthalein. where Acontrol = absorbance of control and Atest = absorbance of sample. The amount of β-cyclodextrin (β-CD) produced was estimated from the standard graph of 0–500 μg/ml β-CD concentration against % decrease in absorbance. One unit of CGTase was defined as the amount of enzyme required to produce 1 μm of β-CD/min. Please can you suggest me the formula for the defination given in the last line
