Answer Posted / himanshu vyas
Autoclave works on Gay Lusaac's Law. If you solve the equation it says Pressure is directly proportional to temperature unlike Boyle's law where its inversely proportional. So as we increase the saturated steam pressure inside the chamber to get 121 degrees Celsius which ultimately increases the temperature of liquid to be sterilized inside the chamber without converting it into steam. (Please refer water phase diagram for better clarity) so due to water phase activity liquid media can attain 121.1 degrees without turning into steam since boiling point of water is 100 degrees. So, as per phase diagram of water if you take the temperature of autoclave chamber to 121.1 degrees by maintaining the chamber pressure at 1.1 bar or 15 PSI, it will serve the purpose of sterilization (Without making the liquid turn into steam). I hope this gives you idea.
| Is This Answer Correct ? | 0 Yes | 0 No |
Post New Answer View All Answers
What are the similarities in the life cycles of the blood fluke and the Chinese liver fluke ?
Which are the various dilutions of sulfuric acid used?
In determining a morph unknown, what tests can I do to narrow between Corynebacterium pseudodiph and xerosis?
Describe the antiviral activity screening facilities ?
How Suspicion of Phage infection occurs?
Describe about pH indicating plates?
What are the conditions of sterilization in an autoclave?
Which are the bacteria or bacterial component that can’t be stained by gram stain?
what is the method for seperartion of spheroplasts?
What are the components of ziehi-neelsen stain?
why OD measurement for bacteria should be under 60 ?
How to differenticate the culture mediums ?
i have rejected once for the reason that why am i not applied for master. as i am already post graduate and i have got i20 for the degree course which is very helpful in my professional career. what should be the best ans to resolve it.
What is the interaction of Group A Streptococcus with HEp-2 & A549 cell line ?
Explain the Lichens Ultrastructure and their physiology ?
