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Why we use caffeine for HPLC calibration in Detector wave
ength accuracy test
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Answer Posted / ajay chansoriya
caffeine having different wavelengths,
1st maxima is 205nm
2nd maxima is 273nm
minima is 245nm
and it is primary reference standard and also suggested in
pharmacopiea.and available easily to market.
Cost is very less, and can be store for long time.
Ajay Chansoriya
Alembic Pharmaceuticals
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