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What is definition of RRT and what is the difference between RT and RRT in HPLC
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Answer Posted / pavana
RT-Retention time (RT) is a measure of the time taken for a solute to pass through a chromatography column. It is calculated as the time from injection to detection. The RT for a compound is not fixed as many factors can influence it even if the same GC and column are used.
RRT-Relative Retention time. relative retention time - ratio between the net retention time of a substance and that of a standard compound. residual silanols - the silanol (-Si-OH) groups that remain on the surface of a silica after a modifier ligands is chemically bonded onto its surface.
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