how calibrate the hplc detector
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Answer Posted / samba siva reddy.p
detector checking with caffiene soln bcz caffiene have 2 max and 1 minima wavelenths,we inject std stock soltn(caffiene soln) with diffrent wavelengths.
201 to 209= 205(maxima)
241 to 249=245(minima)
269 to277=275(maxima)
limits+ or -2
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