why we conclude the area% for the chromatographic purity?

Answer Posted / prakash

1.the absorbance of the analyte peak should not be saturate either below or above 1000 milli absorance Unit.
2. if go for diluted standard level the analyte peak absorbance response should same compare to your high level absorbance response.
3. if you calculate the result either %area normalization or diluted standard level the result should same.
in this case, only we can proceed %area normalization method.

Is This Answer Correct ?    2 Yes 0 No



Post New Answer       View All Answers


Please Help Members By Posting Answers For Below Questions

in api coa contains only process impurities and in product coa contains degradation impurities?

318


Which are the diffrent grades of api in pharma?

311


What is the formula for relative diffrence for standard solution in solution stability in validation?

306


why glutent are detected in the rice cereal baby food product even manufacturer claimed that they are using rice and milk only?we have using ELISA to do the test,and rice supposed not containing any glutent,rite?We already repeat the test so many times and it still detected.just wondering where the glutent came from?

2341


If suppose 10 methods of dissolution given in pharmacopoeial for single content product then which method out of 10? or all 10 require to follow?

527






In which situation we require to prepare the standard solution from sample in Related substance method?

287


before starting analytical method valodation what you checking? and how giving preference to start validation?

344


What if impurity area in control sample coming more as compared to LOQ level of impurity ?

1143


How to calibration of the uv spectroscopy and its test?

669


how require to select dissolution media? what is discrimination?

311


Why we used in n-butyl acetate water content terminology while in ethyl acetate we used moisture content terminology?

1374


what is the different in Total ash, sulphated ash, acid insoluble ash, alcoholic or non alcoholic ash?

9437


How much is the standard area for glc analysis

1744


How to do regeneration of Metacarb Pb plus column?

2077


What is the acceptance criteria for moisture balance when calibrated with sodium sulfate

1545