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How can you calculate manually "Slope" in pH calibration?
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Answer Posted / chmreddy
A good electrode has a mV of 0 ± 30 mV in pH 7.
The slope should be 90 to 105%.
Example:
Readings
pH 4 : 4.29
pH 7 : 7.43
Convert pH 7 reading to mV
7 – 7.43 = -0.43 pH * 59.16 mV/pH = -25.44 (this value is okay because it is within 0 ± 30mV)
Convert pH 4 reading to mV
7 – 4.29 = 2.71 pH * 59.16 mV/pH = 160.32
Calculate slope
160.32 – (-25.44) = 185.76 mV/ 177.48 mV * 100 = 105 %
(this value is borderline as 105% is the upper limit)
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