Why methanol & acetonitrile is mostly used for hplc analysis?
Answer Posted / ankur dwivedi
Most substances are easily soluble in these solvents. They have lower absorbance in routine HPLC working range (205-400 nm). Hence no interference will be there due to these solvents.
Due to less density, they create less pressure in column and hence the column life can be improved.
| Is This Answer Correct ? | 35 Yes | 0 No |
Post New Answer View All Answers
Why only 1.2 million lux hours require in photostability study?
Which products will produce when acetic anhydride reacts with Ter-butanol, Sec-butanol, Iso butanol, Dimethylamine etc.
what type of question will ask in the interview of lab chemist.
What is shaking level in GC?
What is intact assay method development.....?
Why we check moisture,ash & AIA in product
what is definition of validation? which components are followed give detail?
in monograph 7 imp are there but in vendor showing 5 imp do not req to include 2 imp for that what will be justification?
What is Band broading effect explain
why holium oxide prepared in 10% HCLO4 SOLUTION
What is aggregate and fragments in SEC?
Qc interview questions
I have compare C2H2-air and C2H2-N2O flame AAS on determination calcium. Both use same range of std to plot calibration curve. (2-6ppm) When i measure the sample with phosphate, KCl and LaCl, C2H2-N2O flame give false positive result, around 0.5ppm. When i measure the sample with phosphste, KCl and EDTA. C2H2-N2O flame also give 0.5ppm false positive. But both above mentioned sample would not give false positive when measured by C2H2-air flame. What is the reason?
Related substance method equivalency on control sample or spiked sample?
In HPLC Calibration, On which basis RSD Limit of noise test is fixed (NMT 33.0 % )