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Answer Posted / navneet
The main principal of autoclave is when water boiled/heated in a closed container, saturated steam is produced under pressure. According to Boyle's law ,when volume of the steam is kept constant, the temperature is directly proportional to pressure. At 15 lbs pressure 121°C temperature can be obtained. Moist heat coagulates cell protein of the microorganisms and thus kills all the living entities (including spores) in 1in 15-20 min. Hot saturated steam is efficient means of destroying microorganisms, since it penetrate rapidly and gives up its latent heat as it condenses on the cooler object.
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